Abstract
The polypeptide composition of thylakoid membranes of the red alga Cyanidium caldarium was studied by PAGE in the presence of lithium dodecyl sulfate. The thylakoid membranes were shown to contain 65 polypeptides with mol wt from 110 to 10 kDa. PS I isolated from C. caldarium cells is composed of at least 5 components, one of which is the chlorophyll-protein complex with mol wt of 110 kDa typical of higher plants. Cyt f, c 552, b 6 and b 559 were identified. Inhibition of carotenoid biosynthesis with norflurazon caused no changes in the polypeptide composition of thylakoid membranes of the algae grown in dark. The suppression of the biosynthesis rate of some thylakoid polypeptides in the algae grown with norflurazon in light is a result of membrane photodestruction. Thylakoid membranes from C. caldarium cells are more similar in the number of protein components to thylakoid membranes from cells of the cyanobacterium Anacystis nidulans than to those of higher plants (Pisum sativum), which was proved by immune-blotting assays: Thylakoid membranes of the red alga and cyanobacteria contain 28 homologous polypeptides, while thylakoid membranes of the alga and pea, only 15.
Similar content being viewed by others
Abbreviations
- CD:
-
circular dichroism
- CP:
-
chlorophyll-protein complex
- LDS:
-
lithium dodecyl sulfate
- NF:
-
norflurazon
References
Allen MB (1959) Arch Microbiol 32: 270–277
Anderson JM and Levine R (1974) Biochim Biophys Acta 357: 118–126
Bengis C and Nelson N (1977) J. Biol Chem 258: 4564–4569
Bullerjahn GS, Riethman HC and Sherman LA (1985) Biochim Biophys Acta 810: 148–157
Burgess DG and Taylor WC (1987) Planta 170: 520–527
Chua NH and Blomberg F (1979) J Biol Chem 254: 215–223
Dyer TA (1984) In: Baker NR and Barber J (eds) Chloroplast Biogenesis, pp 23–69. Amsterdam: Elsevier Science Publishers
Guikema JA and Sherman LA (1981) Biochim Biophys Acta 637: 189–201
Guikema JA and Sherman LA (1982) Biochim Biophys Acta 681: 440–450
Hladik J and Sofrova D (1981) Photosynthetica 15: 490–503
Hladik J and Sofrova D (1982) Photosynthetica 17: 267–288
Karakashev GV, Stadnichuk IN, Yurina NP and Odintsova MS (1987) Biokhimiya 52: 1485–1493
Karakashev GV, Yurina NP, Shubin VV and Odintsova MS (1988) Biokhimiya 53: 1569–1577
Karapetyan NV, Litvin FF and Krasnovskii AA (1963) Biofizika 8: 191–200
Lundell DJ, Glaser AN and Malkin R (1985) In: Steinback KE, Bonits S, Arntzen CJ and Bogorad L (eds) Molecular Biology of Photosynthetic Apparatus, pp 136–141. Cold Spring Harbor: Cold Spring Harbor Laboratory
Mayfield SP, Nelson T and Taylor WC (1986) Plant Physiol 82: 760–764
Oelmuller R and Mohr H (1986) Planta 167: 106–113
Ortis MN, Melis A and Malkin R (1985) In: Steinback KE, Bonits S, Arntzen CJ and Bogorad L (eds) Molecular Biology of the Photosynthetic Apparatus, pp 130–135. Cold Spring Harbor: Cold Spring Harbor Laboratory
Pakrasi HB, Riethman HC and Sherman LA (1985) Plant Physiol 82: 6903–6908
Redlinger T and Gantt E (1983) Plant Physiol 73: 36–40
Rogner M, Muhlenhoff U, Boekema EJ and Witt HT (1990) Biochim Biophys Acta 1015: 415–424
Schaffner W and Weissmann C (1973) Anal Biochem 56: 502–514
Towbin H, Staehelin T and Gordon J (1979) Proc Natl Acad Sci USA 76: 4350–4354
Vaisberg J and Schiff JA (1976) Plant Physiol 57: 260–269
Vernon LP (1960) Anal Chem 32: 1144–1150
Wettern M and Ohad I (1984) Isr J Bot 33: 253–263
Wettsteinvon D, Möller BL, Hoyer-Hansen G and Simpson D (1982) In: Schiff JA (ed) Origins of Chloroplasts, pp 243–255. Amsterdam: Elsevier
Williams RS and Bennet J (1983) In: Fleischer S and Fleischer B (eds) Methods in Enzymology, pp 487–502 New York: Academic Press
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yurina, N.P., Karakashev, G.V., Karapetyan, N.V. et al. Composition and biosynthesis of thylakoid membrane polypeptides in the red alga Cyanidium caldarium: Comparison with the thylakoid polypeptide composition of higher plants and cyanobacteria. Photosynth Res 30, 15–23 (1991). https://doi.org/10.1007/BF00035678
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00035678