Article

Euphytica

, Volume 85, Issue 1, pp 169-172

Degradation of oxalic acid by transgenic oilseed rape plants expressing oxalate oxidase

  • C. ThompsonAffiliated withPlant Biotechnology, Zeneca Seeds
  • , J. M. DunwellAffiliated withPlant Biotechnology, Zeneca Seeds
  • , C. E. JohnstoneAffiliated withPlant Biotechnology, Zeneca Seeds
  • , V. LayAffiliated withPlant Biotechnology, Zeneca Seeds
  • , J. RayAffiliated withPlant Biotechnology, Zeneca Seeds
  • , M. SchmittAffiliated withAmerican Cyanamid
  • , H. WatsonAffiliated withPlant Biotechnology, Zeneca Seeds
  • , G. NisbetAffiliated withPlant Biotechnology, Zeneca Seeds

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Summary

Oxalic acid is thought to have a primary role in the pathogenicity of several plant pathogens, notably Sclerotinia selerotiorum. A gene coding for the enzyme oxalate oxidase was isolated from barley roots and introduced into oilseed rape as a means of degrading oxalic acid in vivo. This report describes the production of several transgenic plants of oilseed rape and the characterisation of these plants by Southern, Western and enzyme activity assays. Plants were shown to contain an active oxalate oxidase enzyme and were tolerant of exogenously supplied oxalic acid.

Key words

Brassica napus disease tolerance oxalic acid oxalate oxidase Sclerotinia sclerotiorum transformation