Skip to main content
SpringerLink
Log in

Differential expression of tomato (Lycopersicon esculentum L.) genes encoding shikimate pathway isoenzymes. I. 3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase

  • Research Articles
  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

Tomato (Lycopersicon esculentum L. cv. UC82b) was found to contain two distinct 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase genes that are differentially expressed. The corresponding cDNAs were isolated and characterized. Both genes code for putative plastidic DAHP synthase isoforms. The deduced amino acid sequences are 79% identical. A comparison of the known Solanaceae DAHP synthases indicates two distinct conserved isoforms. The steady-state levels of transcripts of the two tomato genes differ in all organs analysed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ausubel, FM, Brent, R, Kingston, RE, Moore, DD, Seidman, JG, Smith, JA, Struhl, K: Current Protocols in Molecular Biology. Greene Publishing Associates/Wiley-Interscience, New York (1987).

    Google Scholar 

  2. Bentley, R: The shikimate pathway—a metabolic tree with many branches. Crit Rev Biochem Mol Biol 25: 307–384 (1990).

    PubMed  Google Scholar 

  3. Boudet, AM: Les acides quinique et shikimique chez les angiospermes arborescentes. Phytochemistry 12: 363–370 (1973).

    Article  Google Scholar 

  4. Dean, C, Tamaki, S, Dunsmuir, P, Favreau, M, Katayama, C, Dooner, H, Bedbrook, J: mRNA transcripts for several higher plant genes are polyadenylated at multiple sites in vivo. Nucl Acids Res 14: 2229–2240 (1986).

    PubMed  Google Scholar 

  5. Doong, RL, Gander, JE, Ganson, RJ, and Jensen, RA: The cytosolic isoenzyme of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase in Spinacia oleracea and other higher plants: extreme substrate ambiguity and other properties. Physiol Plant 84: 351–360 (1992).

    Article  Google Scholar 

  6. Dyer, WE, Henstrand, JM, Handa, AK, Herrmann, KM: Wounding induces the first enzyme of the shikimate pathway in Solanaceae. Proc Natl Acad Sci USA 86: 7370–7373 (1989).

    Google Scholar 

  7. Dyer, WE, Weaver, LM, Zhac, J, Kuhn, DN, Weller, SC, Herrmann, KM: A cDNA encoding 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Solanum tuberosum L. J Biol Chem 265: 1608–1614 (1990).

    PubMed  Google Scholar 

  8. Feinberg, AP, Vogelstein, B: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13 (1983).

    PubMed  Google Scholar 

  9. Ganson, RJ, d'Amato, TA, Jensen, RA: The two-isozyme system of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase in Nicotiana silvestris and other higher plants. Plant Physiol 82: 203–210 (1986).

    Google Scholar 

  10. Gavel, Y, vonHeijne, G: A conserved cleavage-site motif in chloroplast transit peptides. FEBS Lett 261: 455–458 (1990).

    Article  PubMed  Google Scholar 

  11. Görlach J, Schmid J, Amrhein N: Differential expression of tomato (Lycopersicon esculentum L.) genes encoding shikimate pathway isoenzymes. II. Chorismate synthase. Plant Mol Biol, submitted (1993).

  12. Heidecker, G, Messing, J: Structural analysis of plant genes. Annu Rev Plant Physiol 37: 439–466 (1986).

    Google Scholar 

  13. Henikoff, S: Unidirectional digestion with exonuclease III in DNA sequence analysis. Meth Enzymol 155: 156–165 (1987).

    PubMed  Google Scholar 

  14. Henstrand, JM, McCue, KF, Brink, K, Handa, AK, Herrman, KM, Conn, EE: Light and fungal clicitor induce 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase mRNA in suspension cultured cells of parsley (Petroselinum crispum L.). Plant Physiol 98: 761–763 (1992).

    Google Scholar 

  15. Joshi, CP: Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis. Nucl Acids Res 15: 9627–9640 (1987).

    PubMed  Google Scholar 

  16. Keegstra, K, Olsen, LJ, Theg, SM: Chloroplastic precursors and their transport across the envelope membranes. Annu Rev Plant Physiol Plant Mol Biol 40: 471–501 (1989).

    Article  Google Scholar 

  17. Keith, B, Dong, X, Ausubel, FM, Fink, GR: Differential induction of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase genes in Arabidopsis thaliana by wounding and pathogenic attack. Proc Natl Acad Sci USA 88: 8821–8825 (1991).

    Google Scholar 

  18. McCue, KF, Conn, EE: Induction of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase activity by fungal elicitor in cultures of Petroselinum crispum. Proc Natl Acad Sci USA 86: 7374–7377 (1989).

    PubMed  Google Scholar 

  19. Morris, PF, Doong, R-L, Jensen, RA: Evidence from Solanum tuberosum in support of the dual-pathway hypothesis of aromatic biosynthesis. Plant Physiol 89: 10–14 (1989).

    Google Scholar 

  20. Muday, GK, Herrmann, KM: Wounding induces one of two isoenzymes of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase in Solanum tuberosum L. Plant Physiol 98: 496–500 (1992).

    Google Scholar 

  21. Murray, MG, Thompson, WF: Rapid isolation of high molecular weight plant DNA. Nucl Acids Res 8: 4321–4325 (1980).

    PubMed  Google Scholar 

  22. Rubin, JL, Gaines, CG, Jensen, RA: Enzymological basis for herbicidal action of glyphosate. Plant Physiol 70: 833–839 (1982).

    Google Scholar 

  23. Sambrook, J, Fritsch, EF, Maniatis, T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).

    Google Scholar 

  24. Sanger, F, Nicklen, S, Coulson, AR: DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).

    PubMed  Google Scholar 

  25. Schaller, A Schmid, J, Leibinger, U, Amrhein, N: Molecular cloning and analysis of a cDNA coding for chorismate synthase from the higher plant Corydalis sempervirens Pers. J Biol Chem 266: 21434–21438 (1991).

    Google Scholar 

  26. Schmid, J, Schaller, A, Leibinger, U, Boll, W, Amrhein, N: The in vitro synthesized tomato shikimate kinase precursor is enzymatically active and is imported and processed to the mature enzyme by chloroplasts. Plant J 2: 375–383 (1992).

    Article  PubMed  Google Scholar 

  27. Suzich, JA, Dean, JFD, Herrmann, KM: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from carrot root (Daucus carota) is a hysteretic enzyme. Plant Physiol 79: 765–770 (1985).

    Google Scholar 

  28. Ulbrich, B, Zenk, MH: Partial purification and properties of p-hydroxycinnamoyl-CoA: shikimate-p-hydroxy-cinnamoyl transferase from higher plants. Phytochemistry 19: 1625–1629 (1980).

    Article  Google Scholar 

  29. Wang, Y, Herrmann, KM, Weller, SC, Goldsbrough, PB: Cloning and nucleotide sequence of a complementary DNA encoding 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from tobacco. Plant Physiol 97: 847–848 (1991).

    Google Scholar 

  30. Yoshida, SK, Tazaki, K, Minamikawa, T: Occurrence of shikimic and quinic acid in angiosperms. Phytochemistry 14: 195–197 (1975).

    Article  Google Scholar 

  31. Zhao, J, Herrmann, KM: Cloning and sequencing of a second cDNA encoding 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Solanum tuberosum L. Plant Physiol 100: 1075–1076 (1992).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Plant Sciences, Swiss Federal Institute of Technology, Sonneggstrasse 5, CH-8092, Zürich, Switzerland

    Jörn Görlach, Andreas Beck, John M. Henstrand, Jürg Schmid & Nikolaus Amrhein

  2. Department of Horticulture, Purdue University, 47907, West Lafayette, IN, USA

    John M. Henstrand & Avtar K. Handa

  3. Department of Biochemistry, Purdue University, 47907, West Lafayette, IN, USA

    Klaus M. Herrmann

Authors
  1. Jörn Görlach
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Andreas Beck
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. John M. Henstrand
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Avtar K. Handa
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Klaus M. Herrmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jürg Schmid
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Nikolaus Amrhein
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Görlach, J., Beck, A., Henstrand, J.M. et al. Differential expression of tomato (Lycopersicon esculentum L.) genes encoding shikimate pathway isoenzymes. I. 3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase. Plant Mol Biol 23, 697–706 (1993). https://doi.org/10.1007/BF00021525

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00021525

Key words

Search

Navigation