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Aluminum accumulation and associated effects on 15NO3 − influx in roots of two soybean genotypes differing in Al tolerance

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Abstract

A study was conducted to examine aluminum (Al) exclusion by roots of two differentially tolerant soybean (Glycine max L. Merr.) lines, Pl-416937 (Al-tolerant) and Essex (Al-sensitive). Following exposure to 80μM Al for up to 2 h, roots were rinsed with a 10 mM potassium citrate solution and rapidly dissected to allow estimation of intracellular Al accumulation in morphologically distinct root regions. Using 10 min exposures to 300μM 15NO3 − and dissection, accompanying effects on NO3 − uptake were measured. With Al exposures of 20 min or 2 h, there was greater Al accumulation in all root regions of Essex than in those of Pl-416937. The genotypic difference in Al accumulation was particularly apparent at the root apex, both in the tip and in the adjacent root cap and mucilage. Exposure of roots to Al inhibited the uptake of 15NO3 − to a similar extent in all root regions. The results are consistent with Al exclusion from cells in the root apical region being an important mechanism of Al tolerance.

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Authors and Affiliations

  1. Crops Research Laboratory, USDA-ARS, Box 1168, 27565, Oxford, NC, USA

    Dennis B. Lazof & Thomas W. Rufty

  2. Department of Crop Science, North Carolina State University, 27695, NC, Raleigh, USA

    Dennis B. Lazof & Thomas W. Rufty

  3. Department of Biology, Midwestern State University, 763083, Wichita Falls, TX, USA

    Magaly Rincón

  4. USDA-ARS, Department of Agronomy, University of Kentucky, 40546, Lexington, KY, USA

    Charles T. Mackown

  5. USDA-ARS, Soybean and Nitrogen Fixation Research, North Carolina State University, Box 7620, 27695, Raleigh, NC, USA

    Thomas E. Carter

  6. Department of Crop Science, North Carolina State University, Box 7620, 27695, Raleigh, NC, USA

    Thomas E. Carter

Authors
  1. Dennis B. Lazof
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  2. Magaly Rincón
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  3. Thomas W. Rufty
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  4. Charles T. Mackown
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  5. Thomas E. Carter
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Lazof, D.B., Rincón, M., Rufty, T.W. et al. Aluminum accumulation and associated effects on 15NO3 − influx in roots of two soybean genotypes differing in Al tolerance. Plant Soil 164, 291–297 (1994). https://doi.org/10.1007/BF00010081

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  • DOI: https://doi.org/10.1007/BF00010081

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