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Studies on Biosynthesis of Antimicrobial Silver Nanoparticles Using Endophytic Fungi Isolated from the Ethno-medicinal Plant Gloriosa superba L.

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Abstract

Two endophytic fungi isolated from the ethno-medicinal plant Gloriosa superba L. were used for the in vitro biosynthesis of silver nanoparticles (AgNPs). The endophytic fungi were identified as Alternaria solani GS1 and Penicillium funiculosum GS2 based on their ITS regions of rRNA gene sequences. The silver nanoparticles obtained were characterized by UV–visible spectroscopy and transmission electron microscopy. Silver nanoparticles of the size 5–20 nm biosynthesized by A. solani GS1were found to be at peak at 415 nm whereas the AgNPs of the size 5–10 nm biosynthesized using P. funiculosum GS2 showed a maximum peak at 403 nm. An evident superiority of the antimicrobial potency, as denoted by the zone of inhibition by biosynthesized AgNPs using P. funiculosum GS2 compared to that by A. solani GS1, was observed when the nanoparticles were used against three different bacterial strains (Streptococcus pyogenes MTCC1925, Escherichia coli MTCC730 and Enterococcus faecalis MTCC2729) and a fungal strain (Candida albicans MTCC183). The present study elucidates the efficacy of the AgNPs synthesized by endophytic fungi against the three tested bacterial strains as well as the fungal strain C. albicans indicating their potency of bioprospection for medicinal usage.

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Acknowledgments

The financial support received from the Department of Electronics & Information Technology, Govt of India is thankfully acknowledged. The authors are also thankful to the Sophisticated Analytical Instrument Facility (SAIF), North Eastern Hill University, Shillong for providing TEM facilities.

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  1. Microbiology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Umshing, Shillong, 793022, Meghalaya, India

    Lamabam Sophiya Devi, Donald A. Bareh & S. R. Joshi

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  1. Lamabam Sophiya Devi
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  2. Donald A. Bareh
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  3. S. R. Joshi
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Correspondence to S. R. Joshi.

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Devi, L.S., Bareh, D.A. & Joshi, S.R. Studies on Biosynthesis of Antimicrobial Silver Nanoparticles Using Endophytic Fungi Isolated from the Ethno-medicinal Plant Gloriosa superba L.. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 84, 1091–1099 (2014). https://doi.org/10.1007/s40011-013-0185-7

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  • DOI: https://doi.org/10.1007/s40011-013-0185-7

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