Journal of Pharmaceutical Investigation

, Volume 42, Issue 1, pp 33–39

Inhibitory effects of curcuminoids from Curcuma longa on matrix metalloproteinase-1 expression in keratinocytes and fibroblasts

  • Sehyun Jang
  • Jaemoo Chun
  • Eun Myoung Shin
  • Hojeoung Kim
  • Yeong Shik Kim
Research Article

DOI: 10.1007/s40005-012-0005-8

Cite this article as:
Jang, S., Chun, J., Shin, E.M. et al. Journal of Pharmaceutical Investigation (2012) 42: 33. doi:10.1007/s40005-012-0005-8

Abstract

Because aging and inflammation mechanisms share common signaling pathways and mediators, natural products with anti-inflammatory effects are also good candidates as anti-aging agents. Bismethoxycurcumin, demethoxycurcumin, and curcumin are three curcuminoids that can be isolated from Curcuma longa and are well known for their anti-inflammatory and anti-cancer activities. In this study, we examined the anti-wrinkle effects of three curcuminoids from Curcuma longa in keratinocytes and fibroblasts to determine whether an anti-inflammatory agent could also be developed as an anti-aging treatment. The inhibitory effects of UV-B-induced upregulation of nuclear factor-kappaB (NF-κB) activity were detected by a reporter gene assay. Tumor necrosis factor (TNF-α) release was detected by reverse transcriptase-polymer chain reaction (RT-PCR), and matrix metalloprotease-1 (MMP-1) expression was determined by western blot and enzyme-linked immunosorbent assay (ELISA). UV-B induced TNF-α release through NF-κB activation in keratinocytes. Three curcuminoids inhibited the UV-B-induced TNF-α mRNA expression. Specifically, demethoxycurcumin and bisdemethoxycurcunim inhibited UV-B-induced NF-κB activation in HaCaT keratinocytes and reduced the expression of MMP-1 in both keratinocytes and fibroblasts. We found that curcuminoids have anti-wrinkle activity through the inhibition of UV-B induction in keratinocytes and TNF-α induction in fibroblasts. Our results suggest that wrinkle formation and inflammation have common signaling pathways.

Keywords

Curcuminoids TNF-α Matrix metalloproteinase-1 Keratinocytes Fibroblasts 

Copyright information

© The Korean Society of Pharmaceutical Sciences and Technology and Springer Dordrecht 2012

Authors and Affiliations

  • Sehyun Jang
    • 1
  • Jaemoo Chun
    • 1
  • Eun Myoung Shin
    • 1
  • Hojeoung Kim
    • 2
  • Yeong Shik Kim
    • 1
  1. 1.Natural Products Research Institute, College of PharmacySeoul National UniversitySeoulKorea
  2. 2.LG Household and Healthcare R&D CenterDaejeonKorea

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