Infection

, Volume 40, Issue 6, pp 689–694

Dengue virus identification by transmission electron microscopy and molecular methods in fatal dengue hemorrhagic fever

  • D. Limonta
  • V. Falcón
  • G. Torres
  • V. Capó
  • I. Menéndez
  • D. Rosario
  • Y. Castellanos
  • M. Alvarez
  • R. Rodríguez-Roche
  • M. C. de la Rosa
  • A. Pavón
  • L. López
  • K. González
  • G. Guillén
  • J. Diaz
  • M. G. Guzmán
Case Report

DOI: 10.1007/s15010-012-0260-7

Cite this article as:
Limonta, D., Falcón, V., Torres, G. et al. Infection (2012) 40: 689. doi:10.1007/s15010-012-0260-7

Abstract

Dengue virus is the most significant virus transmitted by arthropods worldwide and may cause a potentially fatal systemic disease named dengue hemorrhagic fever. In this work, dengue virus serotype 4 was detected in the tissues of one fatal dengue hemorrhagic fever case using electron immunomicroscopy and molecular methods. This is the first report of dengue virus polypeptides findings by electron immunomicroscopy in human samples. In addition, not-previously-documented virus-like particles visualized in spleen, hepatic, brain, and pulmonary tissues from a dengue case are discussed.

Keywords

Dengue hemorrhagic feverFatalTissuesElectron microscopyRT-PCR

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • D. Limonta
    • 1
  • V. Falcón
    • 2
  • G. Torres
    • 3
  • V. Capó
    • 3
  • I. Menéndez
    • 2
  • D. Rosario
    • 1
  • Y. Castellanos
    • 1
  • M. Alvarez
    • 1
  • R. Rodríguez-Roche
    • 1
  • M. C. de la Rosa
    • 2
  • A. Pavón
    • 1
  • L. López
    • 3
  • K. González
    • 4
  • G. Guillén
    • 5
  • J. Diaz
    • 1
  • M. G. Guzmán
    • 1
  1. 1.Arbovirus Laboratory, Virology Department, PAHO/WHO Collaborating Center for the Study of Dengue and its VectorPedro Kourí Tropical Medicine Institute (IPK)HavanaCuba
  2. 2.Electron Microscopy LaboratoryCenter for Genetic Engineering and Biotechnology (CIGB)HavanaCuba
  3. 3.Pathology DepartmentPedro Kourí Tropical Medicine Institute (IPK)HavanaCuba
  4. 4.Pathology DepartmentSalvador Allende General HospitalHavanaCuba
  5. 5.Biomedical Research DivisionCenter for Genetic Engineering and Biotechnology (CIGB)HavanaCuba