Infection

, Volume 36, Issue 6, pp 597–600

Rapid Diagnosis of CNS Tuberculosis by a T-Cell Interferon-γ Release Assay on Cerebrospinal Fluid Mononuclear Cells

  • K. Kösters
  • R. Nau
  • A. Bossink
  • I. Greiffendorf
  • M. Jentsch
  • M. Ernst
  • S. Thijsen
  • T. Hinks
  • A. Lalvani
  • C. Lange
Case Report

DOI: 10.1007/s15010-007-7316-0

Cite this article as:
Kösters, K., Nau, R., Bossink, A. et al. Infection (2008) 36: 597. doi:10.1007/s15010-007-7316-0

Abstract

Central nervous system tuberculosis remains a clinical diagnostic challenge. The ex vivo Mycobacterium tuberculosis-specific enzyme-linked immunospot assay (ELISPOT) is a novel assay for the rapid detection of M. tuberculosis-specific T-lymphocytes in the peripheral blood. However, when performed on peripheral blood, this assay cannot distinguish between active tuberculosis or latent tuberculosis infection. On the assumption that M. tuberculosis-specific T-lymphocytes migrate to sites of infection, we were able to demonstrate high levels of M. tuberculosis-specific cells by ELISPOT in the cerebrospinal fluid of a patient with tuberculous meningitis and intracerebral tuberculoma four weeks before cerebrospinal fluid culture became positive for M. tuberculosis by culture.

Copyright information

© Springer 2008

Authors and Affiliations

  • K. Kösters
    • 1
  • R. Nau
    • 2
  • A. Bossink
    • 3
  • I. Greiffendorf
    • 1
  • M. Jentsch
    • 1
  • M. Ernst
    • 4
  • S. Thijsen
    • 3
  • T. Hinks
    • 5
  • A. Lalvani
    • 5
  • C. Lange
    • 4
    • 6
  1. 1.Klinikum KrefeldKrefeldGermany
  2. 2.University Hospital of GöttingenGöttingenGermany
  3. 3.Diakonessenhuis UtrechtUtrechtThe Netherlands
  4. 4.Research Center BorstelBorstelGermany
  5. 5.Imperial CollegeLondonUK
  6. 6.Div. Clinical Infectious Diseases, Medical ClinicResearch Center BorstelBorstelGermany