, Volume 36, Issue 6, pp 597–600

Rapid Diagnosis of CNS Tuberculosis by a T-Cell Interferon-γ Release Assay on Cerebrospinal Fluid Mononuclear Cells


  • K. Kösters
    • Klinikum Krefeld
  • R. Nau
    • University Hospital of Göttingen
  • A. Bossink
    • Diakonessenhuis Utrecht
  • I. Greiffendorf
    • Klinikum Krefeld
  • M. Jentsch
    • Klinikum Krefeld
  • M. Ernst
    • Research Center Borstel
  • S. Thijsen
    • Diakonessenhuis Utrecht
  • T. Hinks
    • Imperial College
  • A. Lalvani
    • Imperial College
    • Research Center Borstel
    • Div. Clinical Infectious Diseases, Medical ClinicResearch Center Borstel
Case Report

DOI: 10.1007/s15010-007-7316-0

Cite this article as:
Kösters, K., Nau, R., Bossink, A. et al. Infection (2008) 36: 597. doi:10.1007/s15010-007-7316-0


Central nervous system tuberculosis remains a clinical diagnostic challenge. The ex vivo Mycobacterium tuberculosis-specific enzyme-linked immunospot assay (ELISPOT) is a novel assay for the rapid detection of M. tuberculosis-specific T-lymphocytes in the peripheral blood. However, when performed on peripheral blood, this assay cannot distinguish between active tuberculosis or latent tuberculosis infection. On the assumption that M. tuberculosis-specific T-lymphocytes migrate to sites of infection, we were able to demonstrate high levels of M. tuberculosis-specific cells by ELISPOT in the cerebrospinal fluid of a patient with tuberculous meningitis and intracerebral tuberculoma four weeks before cerebrospinal fluid culture became positive for M. tuberculosis by culture.

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© Springer 2008