, Volume 36, Issue 2, pp 140-146
Date: 12 Mar 2008

Seroepidemiology and Molecular Characterization of Hepatitis E Virus in Jilin, China

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Background:

In order to develop region-specific diagnostic assays and prevent hepatitis E virus (HEV), it is essential to understand epidemiology and genotypic variation within different populations. However, the epidemiological data of HEV infections in Jilin is shortage.

Methods:

The seroepidemiological study was conducted by enzyme-linked immunosorbent assay, and the conserved genomic sequences of open reading frame 2 (348 bp) was detected using reverse transcription-PCR.

Results:

Overall, 1,289 serum samples were positive to HEV-specific immunoglobulin G (IgG), and 180 serum samples were positive to HEV-specific immunoglobulin M (IgM). The seroprevalence of HEV-specific IgG was 26.3%, 27.9%, 25.3%, 32.7% in 2003, 2004, 2005, 2006, respectively. Acute HEV infection mainly occurred in male, people aged from 20 to 69 were more susceptible to infection, and cases with IgM anti-HEV reaction mainly occurred from July to November. HEV RNA was detectable in the serum samples or stool suspension of 15 patients with HEV-specific IgM, and all of these belonged to genotype IV.

Conclusions:

Our results indicate that HEV is widely spread in Jilin and confined to genotype IV.

Dr. G. Zhu and Dr. Y. Qu contributed equally to this work.