Abstract
Genotype along with growing and management conditions can affect the content and the composition of phytochemicals in plants. Two lettuce (Lactuca sativa L.) cultivars, ‘Baronet’ and ‘Red Sails,’ were grown in an open field and high tunnels to examine the effect of growing conditions on their phytochemical content. The total phenolic concentration and antioxidant capacity of lettuce increased in response to transplanting from greenhouse to both open field and high tunnels. However, the increase was much greater when seedlings were transplanted to the open field and was more than 4 fold over the pre-transplant stage. The concentrations of two major phenolic compounds, chicoric acid and chlorogenic acid, were about 2.5–5.5 times higher in both cultivars when grown in open field than in high tunnels. Also, growing lettuce in open field resulted in a greater activation of key genes (phenylalanine ammonia-lyase, L-galactose dehydrogenase and γ-tocopherol methyl transferase) involved in the biosynthesis of phenolic compounds, ascorbic acid and α-tocopherol. ‘Red Sails’ accumulated caffeic acid 4 times as much in open field as it did in high tunnels and overall contained higher amount of phenolic compounds, especially in open field, than did Baronet. Although lettuce plants grown in open field were richer in phytochemicals, a significant reduction in biomass accumulation occurred when the lettuce plants were grown in open field compared to high tunnels regardless of cultivar. These results show that growing conditions, in addition to genotype, can significantly affect the content of many phenolic compounds in lettuce and that growing lettuce under open field can have a positive impact on its health-promoting qualities.
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Literature Cited
Ainsworth, E.A. and K.M. Gillespie. 2007. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nature Protocols 2:875–877.
Altunkaya, A., E.M. Becker, V. Gökmen, and L.H. Skibsted. 2009. Antioxidant activity of lettuce extract (Lactuca sativa) and synergism with added phenolic antioxidants. Food Chem. 115:163–168.
Awika, J.M., L.W. Rooney, X. Wu, R.L. Prior, and L. Cisneros-Zevallos. 2003. Screening methods to measure antioxidant activity of sorghum (Sorghum bicolor) and sorghum products. J. Agric. Food Chem. 51:6657–6662.
Bergmüller, E., S. Porfirova, and P. Dörmann. 2003. Characterization of an Arabidopsis mutant deficient in -tocopherol methyltransferase. Plant Mol. Biol. 52:1181–1190.
Caldwell, C.R. 2003. Alkylperoxyl radical scavenging activity of red leaf lettuce (Lactuca sativa L.) phenolics. J. Agric. Food Chem. 51:4589–4595.
Caldwell, C.R. and S.J. Britz. 2006. Effect of supplemental ultraviolet radiation on the carotenoid and chlorophyll composition of green house-grown leaf lettuce (Lactuca sativa L.) cultivars. J. Food Compos. Anal. 19:637–644.
Chang, S.J., J. Puryear, and J. Cairney. 1993. A simple and efficient method for isolating RNA from pine trees. Plant Mol. Biol. Rep. 11:113–116.
Chappell, J. and K. Hahlbrock. 1984. Transcription of plant defence genes in response to UV light or fungal elicitor. Nature 311:76–78.
Demmig-Adams, B. and W.W. Adams III. 2002. Antioxidants in photosynthesis and human nutrition. Science 298:2149–2153.
Diallinas, G. and A.K. Kanellis. 1994. A phenylalanine ammonia-lyase gene from melon fruit: cDNA cloning, sequence and expression in response to development and wounding. Plant Mol. Biol. 26:473–479.
Dixon, R.A. and N.L. Paiva. 1995. Stress-induced phenylpropanoid metabolism. Plant Cell 7:1085–1097.
García-Macías, P., M. Ordidge, E. Vysini, S. Waroonphan, N.H. Battey, M.H. Gordon, P. Hadley, P. John, J.A. Lovegrove, and A. Wagstaffe. 2007. Changes in the flavonoid and phenolic acid contents and antioxidant activity of red leaf lettuce (Lollo Rosso) due to cultivation under plastic films varying in ultraviolet transparency. J. Agric. Food Chem. 55:10168–10172
Gatzek, S., G.L. Wheeler, and N. Smirnoff. 2002. Antisense suppression of L-galactose dehydrogenase in Arabidopsis thaliana provides evidence for its role in ascorbate synthesis and reveals lights modulated L-galactose synthesis. Plant J. 30:541–553.
Harlan, J.R., 1986. Lettuce and sycamore: sex and romance in ancient Egypt. Eco. Bot. 40:4–15.
Hodges, L. and J.R. Brandle. 1996. Windbreaks: an important component in a plasticulture system. HortTechnology 6:177–181.
Jin, J., O.A. Koroleva, T. Gibson, J. Swanston, J. Magan, Y. Zhang, I.R. Rowland, and C. Wagstaff. 2009. Analysis of phytochemical composition and chemoprotective capacity of rocket (Eruca sativa and Diplotaxis tenuifolia) leafy salad following cultivation in different environments. J. Agric. Food Chem. 57:5227–5234.
Kleinhenz, M.D., D.G. French, A. Gazula, and J.C. Scheerens. 2003. Variety, shading, and growth stage effects on pigment concentrations in lettuce grown under contrasting temperature regimens. HortTechnology 13:677–683.
Lamont, W.J. Jr., M.D. Orzolek, E.J. Holcomb, K. Demchak, E. Burkhart, L. White, and B. Dye. 2003. Production system for horticultural crops grown in the Penn State High Tunnel. Hort-Technology 13:358–362.
Liu, X., S. Ardo, M. Bunning, J. Parry, K. Zhou, C. Stushnoff, F. Stoniker, L. Yu, and P. Kendall. 2007. Total phenolic content and DPPH radical scavenging activity of lettuce (Lactuca sativa L.) grown in Colorado. LWT-Food Sci. Technol. 40:552–557.
Llorach, R., F.A. Tomás-Barberán, and F. Ferreres. 2004. Lettuce and chicory byproducts as a source of antioxidant phenolic extracts. J. Agric. Food Chem. 52:5109–5116.
Llorach, R., A. Martínez-Sánchez, F.A. Tomás-Barberán, M.I. Gil, and F. Ferreres. 2008. Characterisation of polyphenols and antioxidant properties of five lettuce varieties and escarole. Food Chem. 108:1028–1038.
Luthria, D.L., S. Mukhopadhyay, D.T. Krizek. 2006. Content of total phenolics and phenolic acids in tomato (Lycopersicon esculentum Mill.) fruits as influenced by cultivar and solar UV radiation. J. Food Compos. Anal. 19:771–777.
Marchand, L. 2002. Cancer preventive effects of flavonoids-a review. Biomedicine Pharmacotherapy 56:296–301.
Miller, N.J. and C.A. Rice-Evans. 1996. Spectrophotometric determination of antioxidant activity. Redox Rep. 2:161–171.
Nicolle, C., A. Carnat, D. Fraisse, J. Lamaison, E. Rock, H. Michel, P. Amouroux, and C. Remesy. 2004. Characterisation and variation of antioxidant micronutrients in lettuce (Lactuca sativa folium). J. Sci. Food Agric. 84:2061–2069.
Oh, M.-M., H.N. Trick, and C.B. Rajashekar. 2009a. Secondary metabolism and antioxidants are involved in environmental adaptation and stress tolerance in lettuce. J. Plant Physiol. 166: 180–191.
Oh, M.-M., E.E. Carey, and C.B. Rajashekar. 2009b. Environmental stresses induce health-promoting phytochemicals in lettuce. Plant Physiol. Biochem. 47:578–583.
Oh, M.-M., E.E. Carey, and C.B. Rajashekar. 2010. Regulated water deficits improve phytochemical concentration in lettuce. J. Amer. Soc. Hort. Sci. 135:223–229.
Pennycooke, J.C., S. Cox, and C. Stushnoff. 2005. Relationship of cold acclimation, total phenolic content and antioxidant capacity with chilling tolerance in petunia (Petunia × hybrida). Environ. Exp. Bot. 53:225–232.
Rader, H.B. and M.G. Karlsson. 2006. Northern field production of leaf and romaine lettuce using a high tunnel. HortTechnology 16:649–654.
Raviv, M. and Y. Antignus. 2004. UV radiation effects on pathogens and insect pests of greenhouse-grown crops. Photochem. Photobiol. 79:219–226.
Romani, A., P. Pimnelli, C. Galardi, G. Sani, A. Cimato, and D. Heimler. 2002. Polyphenols in greenhouse and open-air-grown lettuce. Food Chem. 79:337–342.
Schmitz-Hoerner, R. and G. Weissenböck. 2003. Contribution of phenolic compounds to the UV-B screening capacity of developing barley primary leaves in relation to DNA damage and repair under elevated UV-B levels. Phytochem. 64:243–255.
Schreiner, M. 2005. Vegetable crop management strategies to increase the quantity of phytochemicals. Eur. J. Nutr. 44:85–94.
Spaw, M. and K. A. Williams. 2004. Full moon farm builds high tunnels: A case study in site planning for crop production structures. HortTechnology 14:449–454.
Tattini, M., L. Guidi, L. Morassi-Bonzi, P. Pinelli, D. Remorini, E. Degl’Innocenti, C. Giordano, R. Massai, and G. Agati. 2005. On the role of flavonoids in the integrated mechanisms of response of Ligustrum vulgare and Phillyrea latifolia to high solar radiation. New Phytol. 167:457–470.
Wells, O.S. 1996. Rowcover and high tunnel growing systems in the United States. HortTechnology 6:172–176.
Wimalasiri, P. and R.B.H. Wills. 1983. Simultaneous analysis of ascorbic acid and dehydroascorbic acid in fruit and vegetables by high-performance liquid chromatography. J. Chromatogr. 256:368–371.
Wu, X., G.R. Beecher, J.M. Holden, D.B. Haytowitz, S.E. Gebhardt, and R.L. Prior. 2004. Lipophilic and hydrophilic antioxidant capacities of common foods in the United States. J. Agric. Food Chem. 52:4026–4037.
Zhao, X., T. Iwamoto, E.E. Carey. 2007. Antioxidant capacity of leafy vegetables as affected by high tunnel environment, fertilisation and growth stage. J. Sci. Food Agric. 87:2692–2699.
Zhao, X., J.R. Nechols, K.A. Williams, W. Wang, and E.E. Carey. 2009. Comparison of phenolic acids in organically and conventionally grown pac choi (Brassica rapa L. chinensis). J. Sci. Food Agric. 89:940–946.
Zhou, Y.-H., Y.Y. Zhang, X. Zhao, H.J. Yu, H.K. Shi, and J.Q. Yu. 2009. Impact of light variation on development of photoprotection, antioxidants, and nutritional value in Lactuca sativa L.. J. Agric. Food Chem. 57:5494–5500.
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Oh, MM., Carey, E.E. & Rajashekar, C.B. Antioxidant phytochemicals in lettuce grown in high tunnels and open field. Hortic. Environ. Biotechnol. 52, 133–139 (2011). https://doi.org/10.1007/s13580-011-0200-y
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DOI: https://doi.org/10.1007/s13580-011-0200-y