Human Cell

, Volume 26, Issue 3, pp 121–127

Establishment and characterization of a novel ovarian clear cell adenocarcinoma cell line, TU-OC-1, with a mutation in the PIK3CA gene

Authors

    • Department of Obstetrics and GynecologyTottori University School of Medicine
  • Misaki Kato
    • Tottori University Hospital Cancer Center
  • Mayumi Nishimura
    • Tottori University Hospital Cancer Center
  • Nao Oumi
    • Tottori University Hospital Cancer Center
  • Tetsuro Oishi
    • Department of Obstetrics and GynecologyTottori University School of Medicine
  • Muneaki Shimada
    • Department of Obstetrics and GynecologyTottori University School of Medicine
  • Shinya Sato
    • Department of Obstetrics and GynecologyTottori University School of Medicine
  • Jun Naniwa
    • Department of Obstetrics and GynecologyTottori University School of Medicine
  • Seiya Sato
    • Department of Obstetrics and GynecologyTottori University School of Medicine
  • Michiko Nonaka
    • Department of Obstetrics and GynecologyTottori University School of Medicine
  • Akiko Kudoh
    • Department of Obstetrics and GynecologyTottori University School of Medicine
  • Naoki Terakawa
    • Department of Obstetrics and GynecologyTottori University School of Medicine
  • Junzo Kigawa
    • Tottori University Hospital Cancer Center
  • Tasuku Harada
    • Department of Obstetrics and GynecologyTottori University School of Medicine
Cell Line

DOI: 10.1007/s13577-013-0062-y

Cite this article as:
Itamochi, H., Kato, M., Nishimura, M. et al. Human Cell (2013) 26: 121. doi:10.1007/s13577-013-0062-y

Abstract

A new cell line of human ovarian clear cell adenocarcinoma (CCC), TU-OC-1, was established and characterized. The cells showed a polygonal-shaped morphology and grew in monolayers without contact inhibition and were arranged like a jigsaw puzzle. The chromosome numbers ranged from 64 to 90. A low rate of proliferation was observed, similar to other CCC cell lines tested (OVTOKO, RMG-I, and OVAS), and the doubling time was 38.4 h. The respective IC50 values of cisplatin and paclitaxel were 12.2 μM and 58.3 nM. Mutational analysis revealed that TU-OC-1 cells harbored a PIK3CA mutation at codon 542 (E542K) in exon 9, which is a mutation hot spot on this gene. We observed that phosphorylated Akt protein was overexpressed in TU-OC-1 cells by western blot analysis. Heterotransplantation to nude mice produced tumors that reflected the original. This cell line may be useful to study the chemoresistant mechanisms of CCC and contribute to novel treatment strategies.

Keywords

Ovarian clear cell adenocarcinomaEstablishmentPIK3CA mutationChemosensitivityMolecular targeted therapy

Copyright information

© Japan Human Cell Society and Springer Japan 2013