Low-dose fractionated radiation with induction chemotherapy for locally advanced head and neck cancer: 5 year results of a prospective phase II trial
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- Gleason, J.F., Kudrimoti, M., Van Meter, E.M. et al. J Radiat Oncol (2013) 2: 35. doi:10.1007/s13566-012-0074-3
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This study aims to report the long-term outcomes of a novel treatment approach utilizing induction low-dose fractionated radiation therapy (LDFRT) and chemotherapy for locally advanced squamous cell carcinoma of head and neck (SCCHN).
We prospectively enrolled 40 patients with locally advanced SCCHN (77 % stage IV) on a phase II clinical trial and treated with induction paclitaxel (225 mg/m2), carboplatin (AUC 6), and LDFRT (80 cGy BID on days 1 and 2) every 21 days for two cycles.
Forty patients enrolled; 39 were evaluable. The acute toxicity and response data have been previously reported; overall response rate (RR) was 82 %. After induction, definitive therapy was concurrent chemoradiation (CRT) in 51 %, XRT alone in 39 %, surgery in 5 %, and surgery and XRT in 5 %. The long-term outcomes are now reported with a median follow-up of 83 months. Locoregional control (LRC) is 80 % and distant control (DC) is 77 %. Five-year overall survival (OS), disease-specific survival, and progression-free survival (PFS) are 62 %, 66 %, and 58 %, respectively.
Induction chemotherapy with LDFRT has a high initial RR, comparable toxicity to two-drug induction regimens, but adds a third novel and effective agent, LDFRT. Five-year follow-up shows favorable outcomes compared to historical controls and excellent compliance with definitive therapy. This novel treatment approach is now planned for phase 3 trial evaluation.
Over 50,000 patients will be diagnosed with squamous cell carcinoma of the head and neck (SCCHN) in the United States in 2011 . Locally advanced disease (stages III, IVA, and IVB) makes up the majority of cases, and historical survival outcomes in these patients range from 30 % to 40 %. Both local and distant recurrences are an issue in this patient population .
Systemic chemotherapy (most frequently platinum-based) has been added to locoregional therapies in an attempt to improve outcomes in these patients. Modern randomized trials have shown that the addition of concurrent chemotherapy to definitive radiation improves locoregional control (LRC), organ preservation, disease-free survival, and in some instances overall survival [3–6]. The addition of concurrent chemotherapy to post-operative radiotherapy has also shown to be beneficial in the high-risk subsets of patients with positive margins and extracapsular extension [7–9]. The results of these and other trials are further bolstered by a large meta-analysis, which at the most recent update, including over 17,000 patients, show a 4.5 % overall survival benefit at 5 years with the addition of chemotherapy to locoregional treatment alone (radiation and/or surgery). The absolute benefit was largest (6.5 %) when the chemotherapy was administered concurrently. Induction chemotherapy also showed a modest improvement in this meta-analysis, 2.4 % at 5 years .
Multiple evidence-based treatment approaches are now available for locally advanced SCCHN. These include surgery followed by radiation (or chemoradiation), definitive chemoradiation, altered fractionation radiation therapy alone, induction chemotherapy followed by chemoradiation or radiation alone, and radiation with concurrent EGFR inhibition. As locoregional control has begun to improve these advances, there has been a renewed interest in induction chemotherapy as a possible way to improve overall survival and decrease the high doses of radiation required currently for definitive management. Spurred by the MACH-NC meta-analysis  showing an overall survival benefit for induction chemotherapy, two recent trials have shown the superiority of taxane-containing induction regimens: TAX 323  and TAX 324  both compared induction TPF (docetaxel, cisplatin, and 5-fluoruracil) versus PF but had different definitive therapies post-induction (radiation in TAX 323 and chemoradiation in TAX 324). TAX 323 demonstrated improvements in progression-free and overall survival at 5 years in the TPF arm , while TAX 324 showed an improvement in 5-year overall survival in the TPF arm [12, 13]. The biggest drawback to the use of induction TPF is the significant acute toxicity (>75 % grade 3 or 4 neutropenia in both trials) of the three-drug combination.
One alternative approach is to utilize low-dose radiation, which has low toxicity, with a two-drug induction regimen. In this way, radiation takes on the role of potentiating the chemotherapy without causing significant toxicity. Low-dose fractionated radiation therapy (LDFRT) (<100 cGy) is a unique biologic phenomenon, characterized by Joiner et al. , who demonstrated that at the lowest doses, hyper-radiosensitivity (HRS) exists . Our institution has reported that LDFRT potentiates the effect of chemotherapy in vitro and in animal models; preclinical data indicate a range of 50 to 80 cGy of low-dose radiation exploits this HRS phenomenon. In addition, four fractions of LDFRT demonstrated maximal cell death when combined with chemotherapy in vitro [16, 17]. This discovery led to the design of the present trial combining the power of LDFRT with induction chemotherapy in locally advanced SCCHN. Induction chemotherapy allowed for rapid assessment of whether HRS was a translatable biologic phenomenon through RR and long-term survival assessment. The initial results of this unique treatment paradigm reported on the response rates and safety using an induction regimen of paclitaxel, carboplatin, and LDFRT , and we now present the long-term follow-up data.
Patient characteristics and eligibility criteria
This was a phase II prospective trial in stage III and IVa/b SCCHN. From July 2000 to May 2002, 40 adult patients were consented per institutional and federal guidelines to receive LDFRT, paclitaxel, and carboplatin (UK H&N-11). Eligible patients had pathologically and radiographically documented locally advanced SCCHN as previously described , and had an Eastern Cooperative Oncology Group (ECOG) performance status of 0–2, and adequate organ function to undergo chemotherapy. No prior history of malignancy within the last 5 years (excluding non-melanomatous skin cancer, or in situ cervix cancer) or pre-existing grade 2 or greater peripheral neuropathy was allowed.
Induction chemotherapy and radiation
Three weeks after the last doses of radiation, patients underwent tumor assessment by pan-endoscopy including primary site assessment by the attending surgeon and radiographic assessment by CT or MRI. Overall response was graded based on the primary and nodal response. Acute toxicity was evaluated using the NCI's Common Toxicity Criteria version 3.0. Late toxicity was determined by review of the patient's medical chart.
Definitive treatment post-induction
Post-induction, the patients were reviewed by a multidisciplinary team including medical oncologists, radiation oncologists, otolaryngologists, radiologists, and pathologists, and post-induction treatment was determined by consensus. Definitive therapy was influenced by response to induction, tolerance of induction, patient wishes, pattern and bulk of residual disease, and medical co-morbidities. As suggested by others [19, 20], patients who had significant responses to induction were treated less intensively with once-daily radiation alone. Patients with bulky residual disease were treated with intensification of therapy with altered fractionation, systemic therapy, or both. All subjects received 3-dimensional planning, and total induction dose of radiation was included into the final dose calculation for definitive therapy. Therefore, the maximum total dose (induction + definitive) for each subject was 7,640 cGy for those receiving once-daily fractionation) or 8,320 cGy for those receiving twice-daily fractionation.
The primary objective of this phase II study was to evaluate antitumor response rates and toxicity of LDFRT and induction chemotherapy in stage III and IVa/b SCCHN as outlined along with design specifications in Arnold et al. . The 5-year rates of progression-free, disease-specific, and overall survival were calculated using the Kaplan–Meier method. Cox proportional hazards models for both overall (OS) and progression-free survival (PFS) were first considered using a saturated model adjusting for variables including gender, age, primary tumor site, tumor category, nodal category, primary treatment, and concurrent chemotherapy. Multivariate models were developed using backward selection to minimize Akaike's information criteria. All statistics were conducted in R .
Follow-up time (years)
Primary tumor site
T1, T2, or T3
Chemo + XRT
Patients were analyzed on an intent-to-treat basis. Thirty-nine patients were evaluable for response, toxicity, local regional control, distant control, and progression-free and overall survival outcomes; one subject was not evaluable because he was lost to follow-up after cycle 1 of induction. Two other patients received one cycle of induction but are included in the outcome analysis; one refused further induction therapy and another progressed in neck nodes. All remaining patients received the full intended dose of induction therapy and only one patient required 1 week delay in administration of cycle 2 secondary to cytopenias.
Acute toxicity and response date
The details of the acute toxicity and response data have been previously reported , with only neutropenia (50 %), infection (8 %), and dermatologic (8 %), grade 3 and 4 toxicity rates over 5 % and no grade 5 toxicities. The response rates (CR + PR) was 82 % overall, with only one subject showing progressive disease (PD) in lymph nodes. A primary site RR of 90 % was observed, with no PD at the primary site.
Definitive therapy began 3 weeks the last dose of chemotherapy and LDFRT in all subjects and was determined by multidisciplinary conference consensus. Definitive therapy consisted of: concurrent chemoradiation in 20 patients (51 %), radiation alone in 15 patients (39 %), surgery in 2 patients (5 %), and surgery and radiation in 2 patients (5 %). Definitive treatment was influenced by response to induction, location of the primary (oral cavity versus other), tolerance of induction, patient wishes, pattern and bulk of residual disease, and medical co-morbidities. Patients who had poor responses to induction were offered surgical therapy or more intensive radiation or chemoradiation. Compliance with definitive therapy was 95 % (38/40 subjects). Radiation was given without treatment interruptions except in two patients. The first patient refused further radiation after 4,260 cGy and underwent salvage surgery, while the second was non-compliant, stopped therapy at 3,880 cGy, and refused further therapy. Five patients underwent adjuvant neck dissections post-radiation with an 80 % pathologic CR rate, and one patient had planned surgery followed by post-operative radiation.
Patients who completed definitive radiation alone received doses ranging from 6,200–7,660 cGy (median 7,200 cGy). Of the 19 patients who received definitive chemoradiation, 16 received hyperfractionated radiation with intra-arterial cisplatin (HYPERRADPLAT) as previously described by Spring et al. in Cancer , while three others received concurrent intravenous chemotherapy.
Of the 39 evaluable patients, 15 are still alive without evidence of disease. Fifteen patients have died of SCCHN, and 9 patients have died of other causes. Fifteen patients recurred: 7 distant only, 6 locoregional only, and 2 failed both distantly and locally. First sites of distant failure were the lungs in 7 patients and multiple simultaneous sites in the other 2 patients. Absolute rates of locoregional control (LRC) and distant control (DC) are 80 % and 77 %, respectively.
Adjusted hazard ratios for overall and progression-free survival
All patients N = 41
Hazard ratioa (95 % CI)
>50 vs. ≤50 years
Primary tumor site
T4 vs. T1–T3
Male vs. female
Primary tumor site
T4 vs. T1–T3
Late toxicity included 4 pts. with feeding tube dependence greater than 1 year, three cases of laryngeal stenosis, two grade 3 esophageal strictures, one osteoradionecrosis, one upper extremity neuropathy, and one tracheocutaneous fistula post-surgery. Second malignancies occurred in 10 (26 %) patients. These included three skin, two head and neck, two lung, one esophageal, one pancreatic, and one rectal cancer.
The initial results of this trial were the first to describe the use of LDFRT as part of a chemotherapy induction regimen for SCCHN in human subjects . Using these very low doses of radiation to enhance the effect of chemotherapy is innovative and expands the range of effective doses of radiotherapy. As elucidated by Joiner et al. , HRS is the phenomenon by which very low radiation doses actually cause increased cell kill per Gray, compared to higher, more conventional doses. Several groups have shown that HRS is independent of DNA-dependent protein kinase complex used to repair double-stranded DNA damage [16, 24]. HRS does not cause initiation of DNA-repair mechanisms that would otherwise arrest these partially damaged cells at the G2 phase of the cell cycle, thus favoring pro-apoptotic pathways . In other words, at the cellular level, without the double-strand breaks caused by standard dose radiation as a trigger, the cell does not recognize the DNA damage from LDRT as significant, does not initiate DNA repair, and the cell dies an apoptotic death because of a lack of recognition of LDRT's cellular damage.
Through the successful addition of LDFRT to induction chemotherapy, we have demonstrated that this novel strategy is tolerable and provides an excellent response rate (82 % overall and 90 % at the primary site). The updated 5-year results presented in this manuscript demonstrate favorable long-term survival outcomes and no unexpected long-term complications when using this unique approach. This would argue against any negative impact from accelerated repopulation when using induction LDFRT. LRC of 80 % and 5 year OS of 62 % compare quite favorably with historical controls [4–6, 25, 26] for treating locally advanced SCCHN. Oral cavity cancers and T4 primary tumors were the factors in our series associated with worse PFS and OS on multivariate analysis.
LDFRT, carboplatin, and paclitaxel had similar toxicities to induction carboplatin and paclitaxel in SCCHN patients as reported by others [27, 28]. LDFRT did not increase the rate of radiation-induced grade 3 or 4 toxicities. In addition, definitive therapy was not delayed or interrupted. When compared to other published trials of induction paclitaxel and carboplatin, our data demonstrates favorable primary site and overall RRs using LDFRT with carboplatin and paclitaxel [18, 27–30].
Our study has limitations in terms of the heterogeneity of post-induction treatment given. While definitive therapy did differ, it was based on stratification by response to induction therapy, an accepted practice advanced by others [19, 20]. The strategy of utilizing response to induction as a guide to the intensity of subsequent therapy did not lead to worsening survival; however, conclusions regarding this approach remain exploratory in nature, because of the small sample size and heterogeneity of the population. We are now able to report the additional endpoint of five-year survival analysis and long-term rates of locoregional and distant control, which are comparable to historic controls. This unique regimen of LDFRT with carboplatin and paclitaxel as an induction does show excellent long-term outcomes, without detriment to survival, or compliance with definitive post-induction therapy, and warrants further investigation.
Comparison of UK H&N 11 regimen to TPF containing induction regimens for locally advanced H&N cancer
Percent stage IV
Grade 3,4 neutropenia
TAX 32311 (n = 177)
37 % (3 years)
TAX 32412,13 (n = 255)
52 % (5 years)
UK H&N 11 (n = 39)
C, P, LDFRT
Radiation, chemoradiation, surgery
62 % (5 years)
Our unique induction regimen of low-dose fractionated radiation, carboplatin, and paclitaxel has shown excellent rates of initial overall response (82 %), 5 year LRC (80 %), and 5 year overall survival (62 %). The acute toxicity profile of this regimen is markedly less than that seen with three-drug induction regimens such as TPF, which have high rates of grade 3 or 4 neutropenia. This may reduce potential delays and alterations in definitive therapy. This long-term follow-up study demonstrates no evidence of diminished therapeutic efficacy with standard platinum-based definitive concurrent chemoradiation following this induction regimen. These results warrant further investigation of LDFRT as part of a systemic induction regimen. That is the focus of investigation for a planned randomized study comparing induction chemotherapy with or without the addition of LDFRT, using the regimen from H&N 11 as the experimental arm. Because we have demonstrated the tolerability of concurrent chemoradiation following this induction regimen, the induction treatment in the randomized trial will be followed by standard platinum-based concurrent chemoradiation in all patients.
In summary, the long-term results using LDFRT in combination with carboplatin and paclitaxel as induction in SCCHN indicate its safety and efficacy with promising overall survival, an excellent rate of locoregional control, and an acceptable distant metastatic rate.
Conflict of interest statement
Bristol-Myers Squibb provided research funds to the University of Kentucky to support this research. Aside from these research funds, provided solely to perform the research in this study, the authors declare that they have no conflict of interest.