Journal of Plant Biochemistry and Biotechnology

, Volume 23, Issue 1, pp 31–41

Salinity mediated biochemical changes towards differential adaptability of three mangroves from Indian Sundarbans

  • Nirjhar Dasgupta
  • Parmita Nandy
  • Chandan Sengupta
  • Sauren Das
Original article

DOI: 10.1007/s13562-012-0182-6

Cite this article as:
Dasgupta, N., Nandy, P., Sengupta, C. et al. J. Plant Biochem. Biotechnol. (2014) 23: 31. doi:10.1007/s13562-012-0182-6

Abstract

Cellular ion homeostasis through osmotic adjustment and efficient free radical scavenging ability are considered as fundamental salt tolerance determinants of plants. Increased salinity (~30 ppt) in Indian Sundarbans has incurred detrimental impact on vegetation pattern, and leading to the precarious existence of some taxa. A comparative account on some physiological and biochemical determinants towards salt stress were assayed in order to focus on the extent of adaptability among the three true mangroves. An in vitro experiment was carried out with mangrove seedlings of Avicennia marina, Bruguiera gymnorrhiza and Heritiera fomes in differential NaCl treatments. Among the five sets of treatment (0, 100, 200, 300 and 400 mM), leaf osmotic potential (ψ) shows consistent hike in A. marina and B. gymnorrhiza along the salinity gradient, at least up to 300 mM (77 and 58 % respectively over control) and comparatively poor in 400 mM. On the other hand, H. fomes reaches its maximum OP value in 200 mM NaCl treatment (64 % increment from control). Thin layer Chromatography was employed for assessing seven free amino acids and spectrophotometric quantification of them from leaf tissues grown in different salinity gradients were performed. The two antioxidative enzymes (Peroxidase and Superoxide dismutase) were estimated qualitatively and quantitatively in three taxa at different salinity level. As compared to other two taxa, H. fomes depicted fewer numbers of isoforms of two enzymes in PAGE analysis, and less enzyme activity across the salinity gradients. Considering the studied parameters, H. fomes might be endorsed towards the feeble adaptability in the present day’s elevated salinity of Indian Sundarbans.

Keywords

Amino acidsAntioxidative enzymesMangrovesOsmotic potentialSundarbans

Abbreviations

PRX

Peroxidase

SOD

Superoxide dismutase

Asp

Aspartic acid

Ala

Alanine

Leu

Leucine

P-Ala

Phenyl-Alanine

Pro

Proline

Trp

Tryptophan

Tyro

Tyrosine

Copyright information

© Society for Plant Biochemistry and Biotechnology 2012

Authors and Affiliations

  • Nirjhar Dasgupta
    • 1
    • 2
  • Parmita Nandy
    • 3
  • Chandan Sengupta
    • 2
  • Sauren Das
    • 1
  1. 1.Agricultural and Ecological Research UnitIndian Statistical InstituteKolkataIndia
  2. 2.Department of BotanyUniversity of KalyaniKalyaniIndia
  3. 3.Department of BotanyBarasat Government CollegeKolkataIndia