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Enzyme-catalyzed hydrolysis of fibre sludge into reducing sugars

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Abstract

The main goal of this research is the enzyme-catalyzed conversion of fibre sludge into reducing sugars, which can further be transformed into higher alcohol biofuels by fermentation. Enzymatic hydrolysis of 1-allyl-3-methylimidazolium chloride ([AMIM]Cl) pretreated fibre sludge demonstrated that this cellulose-rich waste fraction can be successfully converted into reducing sugars. The organic material content of this fibre sludge was determined to be about 80 %. The relative yields of total reducing sugars (glucose) in this study were higher (maximum >95 %) compared with our earlier studies (34 %) of the same pretreatment procedure followed by acid hydrolysis and enzymatic hydrolysis. Due to the ionic liquid used in this study, we were able to recover 85 % (compared with 50 %) of the initial dry fibre sludge with [AMIM]Cl pretreatment.

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Acknowledgments

This work has been carried out within the Forest Refine project (EU Interreg funding 00162639) and SusFuFlex project (Academy of Finland, project nr 124331) which are both gratefully acknowledged. The authors would also like to thank Ms. Anu-Sisko Perttunen for her efforts within this work.

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Authors and Affiliations

  1. Department of Chemistry, University of Oulu, P.O. Box 3000, 90014, Oulu, Finland

    Jana Holm, Ulla Lassi & Johanna Kärkkäinen

  2. Centria University of Applied Sciences, Talonpojankatu 2, 67100, Kokkola, Finland

    Jana Holm & Ganna Zinchenko

  3. Laboratoire de Chimie Moléculaire et Environnement, Université de Savoie, 73376, Le Bourget du Lac Cedex, France

    Audrey Hernoux-Villière

  4. Kokkola University Consortium Chydenius, Talonpojankatu 2 B, 67100, Kokkola, Finland

    Ulla Lassi & Audrey Hernoux-Villière

Authors
  1. Jana Holm
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  2. Ganna Zinchenko
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  3. Ulla Lassi
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  4. Audrey Hernoux-Villière
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  5. Johanna Kärkkäinen
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Correspondence to Ulla Lassi.

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Holm, J., Zinchenko, G., Lassi, U. et al. Enzyme-catalyzed hydrolysis of fibre sludge into reducing sugars. Biomass Conv. Bioref. 3, 353–359 (2013). https://doi.org/10.1007/s13399-013-0082-6

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  • DOI: https://doi.org/10.1007/s13399-013-0082-6

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