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Absorption-Mode Fourier Transform Mass Spectrometry: The Effects of Apodization and Phasing on Modified Protein Spectra

  • Research Article
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Journal of The American Society for Mass Spectrometry

Abstract

The method of phasing broadband Fourier transform ion cyclotron resonance (FT-ICR) spectra allows plotting the spectra in the absorption-mode; this new approach significantly improves the quality of the data at no extra cost. Herein, an internal calibration method for calculating the phase function has been developed and successfully applied to the top-down spectra of modified proteins, where the peak intensities vary by 100×. The result shows that the use of absorption-mode spectra allows more peaks to be discerned within the recorded data, and this can reveal much greater information about the protein and modifications under investigation. In addition, noise and harmonic peaks can be assigned immediately in the absorption-mode.

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Acknowledgments

This work was supported by the University of Warwick, Department of Chemistry, and the Warwick Centre for Analytical Science (EPSRC funded EP/F034210/1). The authors thank Dr. Weidong Cui (Department of Chemistry, Washington University) for helpful discussion.

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Correspondence to Peter B. O’Connor.

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Qi, Y., Li, H., Wills, R.H. et al. Absorption-Mode Fourier Transform Mass Spectrometry: The Effects of Apodization and Phasing on Modified Protein Spectra. J. Am. Soc. Mass Spectrom. 24, 828–834 (2013). https://doi.org/10.1007/s13361-013-0600-6

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  • DOI: https://doi.org/10.1007/s13361-013-0600-6

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