Research Article

Journal of The American Society for Mass Spectrometry

, Volume 23, Issue 6, pp 1062-1070

Surface Acoustic Wave Nebulization Produces Ions with Lower Internal Energy than Electrospray Ionization

  • Yue HuangAffiliated withDepartment of Chemistry, University of Washington
  • , Sung Hwan YoonAffiliated withDepartment of Medicinal Chemistry, University of Washington
  • , Scott R. HeronAffiliated withDepartment of Medicinal Chemistry, University of Washington
  • , Christophe D. MasselonAffiliated withDepartment of Medicinal Chemistry, University of WashingtonCommissariat à l’Énergie Atomique
  • , J. Scott EdgarAffiliated withDepartment of Medicinal Chemistry, University of Washington
  • , František TurečekAffiliated withDepartment of Chemistry, University of Washington
  • , David R. GoodlettAffiliated withDepartment of Medicinal Chemistry, University of Washington Email author 

Abstract

Surface acoustic wave nebulization (SAWN) has recently been reported as a novel method to transfer non-volatile analytes directly from solution to the gas phase for mass spectrometric analysis. Here we present a comparison of the survival yield of SAWN versus electrospray ionization (ESI) produced ions. A series of substituted benzylpyridinium (BzPy) compounds were utilized to measure ion survival yield from which ion energetics were inferred. We also estimated bond dissociation energies using higher level quantum chemical calculations than previously reported for BzPy ions. Additionally, the effects on BzPy precursor ion survival of SAWN operational parameters such as inlet capillary temperature and solution flow-rate were investigated. Under all conditions tested, SAWN-generated BzPy ions displayed a higher tendency for survival and thus have lower internal energies than those formed by ESI.

Key words

Surface acoustic wave nebulization Internal energy Ionization method ESI