Focus in Honor of David Muddiman, Recipient of the 2010 Biemann Medal
The 2010 Biemann Medal was awarded to Professor David C. Muddiman at the 58th annual ASMS Conference for his numerous research contributions to interdisciplinary bioanalytical mass spectrometry. This rather broad focus issue reflects Dave’s own diverse research interests in the development of mass spectrometric instrumentation, methodologies, and their applications to biological problems as well as his long-term commitment to collaborative, cross-cutting research. In particular, Dave has focused on innovative ways to create and manipulate ions to extend the usefulness of mass spectrometry by including new ionization methods for imaging mass spectrometry and improving ionization efficiency. These advances will ultimately allow for a greater understanding of the biology underlying complex molecular processes.
This Special Focus Issue on “Bioanalytical Mass Spectrometry” is dedicated to Dave Muddiman. This issue opens with an Account and Perspective of John Fenn authored by Michael Grayson. John Fenn was one of Dave’s special mentors due to their extensive interactions while on the faculty at Virginia Commonwealth University; John’s broad outlook and passion for innovation inspired Dave’s development as a leader in the field of mass spectrometry. This focus also contains original contributions from eight groups, including one by Dave’s own research group. Dave and his co-authors report the synthesis and characterization of a series of hydrophobic reagents for derivatization of N-linked glycans and enhancement of their ion abundances upon electrospray ionization. In the third article, Richard D. Smith and co-workers describe the integration of subambient pressure nanoelectrospray ionization source with gradient reversed-phase liquid chromatography, affording high sensitivity for complex mixtures like tryptic digests. Graham Cooks and co-authors report the development of desorption electrospray ionization (DESI) imaging for characterization of human seminoma tissue based on the glycerophospholipid profiles. Ljiljana Pasa-Tolic and co-workers calculate the electric field inside several FT-ICR cells and evaluate the outcome of cell compensation and impact on cell design. Kermit Murray and co-workers describe the use of an IR laser to ablate biological molecules from samples (blood, egg, milk) under ambient conditions, followed by capture of the ablated molecules in solvent droplets that are either deposited on a target and subsequently analyzed by MALDI or are injected into a nanoelectrospray source. Alan Marshall and co-workers present a re-designed FT-ICR mass spectrometer with improved conductance, better ion optical alignment, enhanced ion transmission, and reduced detection circuit capacitance, ultimately leading to a two-fold improvement in sensitivity. Pat Limbach and co-workers report an LC-MS/MS method and selected reaction monitoring assay that enhances the identification and quantitative analysis of pseudouridine in RNA, as demonstrated for the incorporation of pseudouridine in the conserved T-loop of E. coli tRNA. In the final article of the special focus, Matthias Mann and co-workers report an innovative recalibration function that in some cases outperforms the use of a standard lock mass for improvement of mass accuracy in proteomics experiments utilizing an LTQ-Orbitrap mass spectrometer.
Dave is a rising star whose contributions to bioanalytical mass spectrometry have culminated in his selection as the recipient of the 2010 Biemann Medal. The Journal of the American Society for Mass Spectrometry is honored to present this focus section to highlight Dave’s contributions and the significant impact of his interdisciplinary approach.