, Volume 6, Issue 1, pp 87-90
Date: 03 Nov 2011

First report of Phoma clematidina the cause of leaf spot-wilt disease of Clematis pubescens in Australia


Leaf lesions and wilt of Clematis pubescens were observed in the Jarrah (Eucalyptus marginata) forest of South-Western Australia in 2009. A Phoma sp. was consistently isolated from the infected tissues and its morphological characteristics were determined. The identity of the fungus was confirmed by sequencing of the internal transcribed spacer (ITS) region of the rDNA operon and Phoma clematidina was identified. A pathogenicity test was conducted and Koch’s postulates were fulfilled by re-isolation of the identified fungus. This is the first report of P. clematidina causing leaf lesions and wilt of Clematis pubescens in Australia.