Tumor Biology

, Volume 37, Issue 7, pp 8765–8773

Inhibition of miR-15b decreases cell migration and metastasis in colorectal cancer

  • Jian Li
  • Yuxiang Chen
  • Xiong Guo
  • Ling Zhou
  • Zeming Jia
  • Yaping Tang
  • Ling Lin
  • Weidong Liu
  • Caiping Ren
Original Article

DOI: 10.1007/s13277-015-4396-9

Cite this article as:
Li, J., Chen, Y., Guo, X. et al. Tumor Biol. (2016) 37: 8765. doi:10.1007/s13277-015-4396-9

Abstract

Colorectal cancer (CRC) has a high prevalence and mortality rate. Biomarkers for predicting the recurrence of CRC are not clinically available. This study investigated the role of circulating miR-15b in the prediction of CRC recurrence and the associated mechanism. miR-15b levels in plasma and tissues were measured by real-time PCR. Metastasis suppressor-1 (MTSS1) and Klotho protein expression were detected by Western blot and immunohistochemistry. Invasion and migration of CRC tumor cells were measured by transwell plates. Liver metastasis was established by intraspleen injection of HCT116 cells. Plasma miR-15b levels were significantly higher in CRC patients than in healthy controls, in CRC patients with metastasis than in CRC patients without metastasis, and in CRC patients with recurrence than in CRC patients without recurrence in the 5-year follow-up. miR-15b level in CRC tumors was significantly higher than that in peritumoral tissues. High plasma miR-15b level and negative MTSS1 and Klotho expression in tumor tissues significantly correlated with poor survival. Inhibition of miR-15b activity by adenovirus carrying antimiR-15b sequence significantly increased MTSS1 and Klotho protein expression and subsequently decreased colony formation ability, invasion, and migration of HCT116 cells in vitro and liver metastasis of HCT116 tumors in vivo. In conclusion, high abundance of circulating miR-15b correlated with tumor metastasis, recurrence, and poor patient prognosis through downregulation of MTSS1 and Klotho protein expression.

Keywords

Colorectal cancer Metastasis miR-15b Prognosis MTSS1 Klotho 

Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Jian Li
    • 1
  • Yuxiang Chen
    • 1
  • Xiong Guo
    • 1
  • Ling Zhou
    • 1
  • Zeming Jia
    • 1
  • Yaping Tang
    • 1
  • Ling Lin
    • 1
  • Weidong Liu
    • 2
  • Caiping Ren
    • 2
  1. 1.Hepatobiliary and Enteric Surgery Research Center, Xiangya HospitalCentral South UniversityChangshaPeople’s Republic of China
  2. 2.Cancer Research Institute, Collaborative Innovation Center for Cancer Medicine, Key Laboratory for Carcinogenesis of Chinese Ministry of Health, School of Basic Medical ScienceCentral South UniversityChangshaPeople’s Republic of China