Tumor Biology

, Volume 37, Issue 4, pp 5385–5395

STAT3:FOXM1 and MCT1 drive uterine cervix carcinoma fitness to a lactate-rich microenvironment

  • Lidia Santos Silva
  • Luis Gafeira Goncalves
  • Fernanda Silva
  • Germana Domingues
  • Valdemar Maximo
  • Joana Ferreira
  • Eric W.-F. Lam
  • Sergio Dias
  • Ana Felix
  • Jacinta Serpa
Original Article

DOI: 10.1007/s13277-015-4385-z

Cite this article as:
Silva, L.S., Goncalves, L.G., Silva, F. et al. Tumor Biol. (2016) 37: 5385. doi:10.1007/s13277-015-4385-z
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Abstract

Uterine cervix cancer is the second most common malignancy in women worldwide with human papillomavirus (HPV) as the etiologic factor. The two main histological variants, squamous cell carcinomas (SCC) and adenocarcinomas (AC), resemble the cell morphology of exocervix and endocervix, respectively. Cancer metabolism is a cancer hallmark conditioned by the microenvironment. As uterine cervix homeostasis is dependent on lactate, we hypothesized lactate plays a role in uterine cervix cancer progression. Using in vitro (SiHa-SCC and HeLa-AC) and BALB-c/SCID models, we demonstrated that lactate metabolism is linked to histological types, with SCC predominantly consuming and AC producing lactate. MCT1 is a key factor, allowing lactate consumption and being regulated in vitro by lactate through the FOXM1:STAT3 pathway. In vivo models showed that SCC (SiHa) expresses MCT1 and is dependent on lactate to grow, whereas AC (HeLa) expresses MCT1 and MCT4, with higher growth capacities. Immunohistochemical analysis of tissue microarrays (TMA) from human cervical tumors showed that MCT1 expression associates with the SCC type and metastatic behavior of AC, whereas MCT4 expression concomitantly increases from in situ SCC to invasive SCC and is significantly associated with the AC type. Consistently, FOXM1 expression is statistically associated with MCT1 positivity in SCC, whereas the expression of FOXO3a, a FOXM1 functional antagonist, is linked to MCT1 negativity in AC. Our study reinforces the role of the microenvironment in the metabolic adaptation of cancer cells, showing that cells that retain metabolic features of their normal counterparts are positively selected by the organ’s microenvironment and will survive. In particular, MCT1 was shown to be a key element in uterine cervix cancer development; however, further studies are needed to validate MCT1 as a suitable therapeutic target in uterine cervix cancer.

Keywords

Uterine cervix cancer MCT1 Metabolic symbiosis STAT3-FOXM1 New therapeutic target 

Supplementary material

13277_2015_4385_MOESM1_ESM.docx (17 kb)
Supplementary Table 1(DOCX 16 kb)
13277_2015_4385_MOESM2_ESM.docx (183 kb)
Supplementary Figure 1(DOCX 182 kb)

Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Lidia Santos Silva
    • 1
    • 2
  • Luis Gafeira Goncalves
    • 3
  • Fernanda Silva
    • 1
    • 2
  • Germana Domingues
    • 1
    • 2
  • Valdemar Maximo
    • 4
    • 5
  • Joana Ferreira
    • 2
  • Eric W.-F. Lam
    • 6
  • Sergio Dias
    • 7
  • Ana Felix
    • 1
    • 2
  • Jacinta Serpa
    • 1
    • 2
  1. 1.Centro de Estudos de Doenças Crónicas (CEDOC), NOVA Medical School/Faculdade de Ciências MédicasUniversidade Nova de LisboaLisbonPortugal
  2. 2.Instituto Português de Oncologia de Lisboa Francisco Gentil (IPOLFG)LisbonPortugal
  3. 3.Instituto de Tecnologia Quimica e Biológica (ITQB) António XavierUniversidade NOVAOeirasPortugal
  4. 4.Medical FacultyUniversity of PortoPortoPortugal
  5. 5.Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP)PortoPortugal
  6. 6.Department of Surgery and CancerImperial College LondonLondonUK
  7. 7.Instituto de Medicina Molecular da Universidade de LisboaLisbonPortugal

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