Tumor Biology

, Volume 36, Issue 10, pp 8015–8023

MicroRNA-218 inhibits bladder cancer cell proliferation, migration, and invasion by targeting BMI-1

  • Yidong Cheng
  • Xiao Yang
  • Xiaheng Deng
  • Xiaolei Zhang
  • Pengchao Li
  • Jun Tao
  • Qiang Lu
Research Article

DOI: 10.1007/s13277-015-3532-x

Cite this article as:
Cheng, Y., Yang, X., Deng, X. et al. Tumor Biol. (2015) 36: 8015. doi:10.1007/s13277-015-3532-x

Abstract

MicroRNAs (miRNAs) are recognized as important molecules and have emerged as important gene regulators in tumorigenesis. Growing evidence suggested that miR-218 was a tumor suppressor in many human cancers. However, its underlying role in bladder cancer (BCa) remains unclear. The aim of this study was to explore the effect of miR-218 on the proliferation, migration, and invasion of BCa cells. We found that miR-218 was frequently downregulated in BCa tissues compared with normal adjacent tissues. In vitro and in vivo assays demonstrated that miR-218 overexpression in the BCa cells inhibited cell proliferation, migration, and invasion. Luciferase reporter assay showed that BMI-1 was a direct target of miR-218. In addition, we found that miR-218 regulated the expression of BMI-1 and its downstream target (PTEN) and participated in the phosphorylation of AKT. Our findings indicate that miR-218 functions as tumor suppressor in BCa, and the miR-218/BMI-1 axis may provide novel diagnostic and therapeutic strategies for the treatment of BCa.

Keywords

MicroRNA-218 Bladder cancer BMI-1 Proliferation Migration Invasion 

Supplementary material

13277_2015_3532_MOESM1_ESM.doc (52 kb)
ESM 1(DOC 51 kb)

Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Yidong Cheng
    • 1
  • Xiao Yang
    • 1
  • Xiaheng Deng
    • 1
  • Xiaolei Zhang
    • 1
  • Pengchao Li
    • 1
  • Jun Tao
    • 1
  • Qiang Lu
    • 1
  1. 1.Department of UrologyThe First Affiliated Hospital of Nanjing Medical UniversityNanjingChina

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