Tumor Biology

, Volume 36, Issue 10, pp 7831–7840

MicroRNA-25 promotes gastric cancer proliferation, invasion, and migration by directly targeting F-box and WD-40 Domain Protein 7, FBXW7

  • Junhua Gong
  • Zheng Cui
  • Li Li
  • Qiang Ma
  • Qiufang Wang
  • Yinhe Gao
  • Hao Sun
Research Article

DOI: 10.1007/s13277-015-3510-3

Cite this article as:
Gong, J., Cui, Z., Li, L. et al. Tumor Biol. (2015) 36: 7831. doi:10.1007/s13277-015-3510-3

Abstract

Increasing evidence shows that abnormal microRNA (miRNA) expression is involved in tumorigenesis. MiR-25 was previously reported to act as tumor suppressor or oncogene in diverse cancers. However, their expression, function, and mechanism in gastric cancer (GC) are not well known. Here, we show that miR-25 was overexpressed in primary tumor tissues of GC patients and was significantly correlated with a more aggressive phenotype of GC in patients. MiR-25 inhibition significantly decreased the proliferation, invasion, and migration of GC cells in vitro. Furthermore, miR-25 repressed F-box and WD-40 domain protein 7 (FBXW7) expression by directly binding to 3-untranslated region (UTR) of FBXW7, and the inverse correlation was observed between the expressions of miR-25 and FBXW7 mRNA in primary GC tissues. Moreover, the restoration of FBXW7 led to suppressed proliferation, invasion, and migration of GC cells. In vivo, miR-25 promotes tumor growth of GC. Taken together, miR-25 promotes GC progression by directly downregulating FBXW7 expression and may be employed as a novel prognostic marker and therapeutic target of GC.

Keywords

MicroRNA-25FBXW7Gastric cancerProliferationInvasionMigration

Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Junhua Gong
    • 1
  • Zheng Cui
    • 1
  • Li Li
    • 1
  • Qiang Ma
    • 1
  • Qiufang Wang
    • 1
  • Yinhe Gao
    • 1
  • Hao Sun
    • 1
  1. 1.Health checkup CenterBeidaihe Sanatorium of Beijing Military Area Command, Chinese PLAQinhuangdaoPeople’s Republic of China