Tumor Biology

, Volume 36, Issue 9, pp 6805–6811

miR-30 overexpression promotes glioma stem cells by regulating Jak/STAT3 signaling pathway

  • Shusheng Che
  • Tingting Sun
  • Jianpeng Wang
  • Yingbin Jiao
  • Chao Wang
  • Qinghai Meng
  • Weiguo Qi
  • Zhiyong Yan
Research Article

DOI: 10.1007/s13277-015-3400-8

Cite this article as:
Che, S., Sun, T., Wang, J. et al. Tumor Biol. (2015) 36: 6805. doi:10.1007/s13277-015-3400-8

Abstract

Malignant glioma is the most common intracranial tumor with poor prognosis. It is well believed that glioma stem cells (GSCs) are responsible for the initiation and progression of glioma. Janus kinase/signal transducer and activator of transcription (Jak/STAT3) pathway plays a key role in the functions of GSCs. However, the regulatory mechanism of Jak/STAT3 pathway has not been completely elucidated. This study employed multidisciplinary approaches to investigate the upstream regulators of Jak/STAT3 signaling in GSCs. miR-30 was found to be overexpressed in the GSCs derived from U-87 MG and primary glioma cells, compared with non-stem-cell-like glioma cells and normal cells. Downregulation of miR-30 was able to suppress Jak/STAT3 pathway and reduce the tumorigenecity of GSCs. miR-30 decreased the expression of suppressor of cytokine signaling 3 (SOCS3) expression by targeting 3′UTR of its mRNA. The silencing of SOCS3 abolished the effect of miR-30 downregulation on GSCs. Collectively, there is a regulatory pathway consisting of miR-30, SOCS3, and Jak/STAT3 in GSCs, and targeting this pathway may be a promising strategy to treat glioma.

Keywords

miR-30SOCS3Jak/STAT3GliomaGSC

Supplementary material

13277_2015_3400_Fig5_ESM.jpg (148 kb)
ESM 1

(JPEG 148 kb)

13277_2015_3400_MOESM1_ESM.pptx (93 kb)
ESM 2(PPTX 93 kb)
13277_2015_3400_MOESM2_ESM.docx (28 kb)
ESM 3(DOCX 28 kb)

Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Shusheng Che
    • 1
  • Tingting Sun
    • 2
  • Jianpeng Wang
    • 1
  • Yingbin Jiao
    • 1
  • Chao Wang
    • 1
  • Qinghai Meng
    • 1
  • Weiguo Qi
    • 1
  • Zhiyong Yan
    • 1
  1. 1.Department of Neurosurgerythe Affiliated Hospital of Qingdao UniversityQingdaoChina
  2. 2.Intensive Care UnitQingdao Municipal HospitalQingdaoChina