Tumor Biology

, Volume 35, Issue 6, pp 5339–5345

Clinical significance of the stem cell gene Oct-4 in cervical cancer

Research Article

DOI: 10.1007/s13277-014-1696-4

Cite this article as:
Yang, Y., Wang, Y., Yin, C. et al. Tumor Biol. (2014) 35: 5339. doi:10.1007/s13277-014-1696-4

Abstract

This study aims to investigate the association between the expression of Oct-4 and the biological behavior or prognosis of cervical cancer. Serum-free suspension culture technology was used to select a suspension of microspheres that can stabilize clones. The tumorigenicity of the microsphere suspension was analyzed in NOD/SCID mice. Microarray analysis was used to detect the specific expression of genes in the microsphere suspension. The expression of Oct-4 was detected by immunohistochemistry, and the correlation between Oct-4 expression and clinical pathological prognostic indicators was analyzed in cervical cancer. The expression of the following genes was significantly different between the experimental and control groups: stem cell differentiation (CD44 and Oct-4), markers cell cycle regulators (APC), cell cycle regulators (MYC), and self-renewal markers (MYST2, NEUROG2, and SOX1). The expression of Oct-4 was significantly higher in cervical cancer tissues than in adjacent normal tissues and was significantly related to differentiation, clinical stage, and lymph node metastasis. The 5-year survival rate of patients with Oct-4-positive expression was lower than that of patients with Oct-4-negative expression (36.7 vs. 67.7 %, respectively; P = 0.001). Cox regression analysis revealed that clinical stage, lymph node metastasis, and Oct-4 were independent prognostic factors in cervical cancer (P = 0.031, 0.012, and 0.001, respectively). Our results showed that Oct-4 was highly expressed in cervical cancer stem cells; Oct-4 expression was associated with biological behavior and was an independent prognostic factor in cervical cancer. Therefore, it may represent a potential target for cervical cancer treatment.

Keywords

Cervical cancer Stem cell Oct-4 Relapse Survival 

Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Yanyan Yang
    • 1
  • Yimin Wang
    • 1
  • Chunxia Yin
    • 2
  • Xiuying Li
    • 1
  1. 1.Research CentreChina-Japan Union Hospital of Jilin UniversityChangchunPeople’s Republic of China
  2. 2.Changchun Obstetrics-Gynecology HospitalChangchunPeople’s Republic of China

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