Tumor Biology

, Volume 34, Issue 6, pp 3561–3567

CYP1B1 C4326G polymorphism and susceptibility to cervical cancer in Chinese Han women

  • Ya Li
  • Shi-Qiao Tan
  • Qian-Hong Ma
  • Lei Li
  • Zhong-Ying Huang
  • Yan Wang
  • Shang-Wei Li
Research Article

DOI: 10.1007/s13277-013-0935-4

Cite this article as:
Li, Y., Tan, S., Ma, Q. et al. Tumor Biol. (2013) 34: 3561. doi:10.1007/s13277-013-0935-4
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Abstract

Cytochrome P450 1B1 (CYP1B1) is a key P450 enzyme, which could catalyze the formation of 4-hydroxy estrogen metabolites and play a role in estrogen-dependent cancers. We hypothesized that genetic variant in CYP1B1 may modify individual susceptibility to cervical cancer. The aim of this study was to evaluate the association between CYP1B1 C4326G polymorphism and cervical cancer risk in Chinese women. We extracted the peripheral blood samples in 250 patients with cervical cancer and 250 female controls. The matrix-assisted laser desorption ionization time-of-flight mass spectrometry method and direct DNA sequencing were performed to detect the polymorphism. The frequencies of CC, CG, and GG genotypes of CYP1B1 C4326G in cases and controls were 66.0, 26.8, 7.2 % and 75.2, 21.6, and 3.2 %, respectively, and there was a significant difference between the two groups (P = 0.034). Compared with the wild-type CC genotype, the variant GG genotype was associated with a significantly increased risk of cervical cancer (adjusted OR = 2.30; 95 % CI = 1.02, 5.50). Moreover, stratification analysis by age, smoking, drinking, human papillomaviruses (HPV) 16 or 18 carrier status, and family history of cervical cancer, we found that the variant genotypes containing the G allele were associated with a significantly increased risk of cervical cancer among HPV 16 or 18-positive individuals (adjusted OR = 2.85; 95 % CI = 1.45, 5.62) and among women younger than 45 years old (adjusted OR = 1.87; 95 % CI = 1.03, 3.37). These results suggest that CYP1B1 C4326G polymorphism may increase risk of cervical cancer in Chinese women, especially among young individuals with high-risk HPV infection.

Keywords

Cervical cancerCYP1B1PolymorphismCase–control study

Abbreviations

CYP1B1

Cytochrome P450 1B1

OR

Odds ratio

CI

Confidence interval

PCR

Polymerase chain reaction

SNP

Single nucleotide polymorphisms

HWE

Hardy–Weinberg equilibrium

Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  • Ya Li
    • 1
  • Shi-Qiao Tan
    • 2
  • Qian-Hong Ma
    • 1
  • Lei Li
    • 1
  • Zhong-Ying Huang
    • 1
  • Yan Wang
    • 1
  • Shang-Wei Li
    • 1
  1. 1.Division of Reproductive Medical CenterWest China Second University Hospital, Sichuan UniversityChengduChina
  2. 2.Division of Reproductive Endocrinology and InfertilityWest China Second University Hospital, Sichuan UniversityChengduChina