Isolation of hyperthermal stress responsive genes in soft coral ( Scleronephthya gracillimum)
First Online: 30 December 2010 Received: 17 February 2010 Accepted: 08 September 2010 DOI:
Cite this article as: Woo, S., Jeon, H., Lee, J. et al. Mol. Cell. Toxicol. (2010) 6: 384. doi:10.1007/s13273-010-0051-0 Abstract
The extensive isolation of genes responsive to hyperthermal stress conditions in soft coral (
Scleronephthya gracillimum) is described. Soft coral colonies were exposed to high seawater temperature conditions. Gene candidates whose transcript levels changed in response to hyperthermal conditions were identified by differential display polymerase chain reaction (DD-PCR). Twenty-four types of candidate genes were identified, 18 of which were upregulated in expression and 6 of which were downregulated. The genes were found to function in post-translational modification, protein turnover and chaperones (O); translation, ribosomal structure and biogenesis (J); signal transduction mechanisms (T); defense mechanisms (V); inorganic ion transport and metabolism (P); energy production and conversion (C); cytoskeleton (Z); cell cycle control, cell division and chromosome partitioning (D); lipid transport and metabolism (I); chromatin structure and dynamics (B); transcription (K); replication, recombination and repair (L); secondary metabolites biosynthesis, transport and catabolism (Q); extracellular structures (W); general function prediction (R); and finally, unknown function (S) based on KOG classification. Among these candidates, their expressional changes were confirmed by real-time quantitative PCR (qRT-PCR). These 24 isolated gene candidates were differentially expressed and therefore have great potential as molecular biomarkers for the identification of environmental stressors. Keywords Hyperthermal stress Differentially expressed genes Real-time quantitative PCR Soft coral Scleronephthya gracillimum References
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