Genes & Genomics

, Volume 36, Issue 3, pp 303–311

Identification of a pollen-specific gene, SlCRK1 (RFK2) in tomato

  • Woong Bom Kim
  • So Young Yi
  • Sang-Keun Oh
  • Chan Ju Lim
  • Hyun A. Kim
  • Hyun A. Jang
  • Ha Yeon Lee
  • Youn-Il Park
  • Suk-Yoon Kwon
Research Article

DOI: 10.1007/s13258-013-0168-3

Cite this article as:
Kim, W.B., Yi, S.Y., Oh, SK. et al. Genes Genom (2014) 36: 303. doi:10.1007/s13258-013-0168-3

Abstract

Plant receptor-like kinases (RLKs) are proteins that are involved in the regulation of development, hormone signaling, abiotic, and biotic stress responses. It has been suggested that cysteine-rich receptor-like kinases (CRKs), which are one of the largest RLK groups, is significant in pathogen defense and programmed cell death. The CRK1 gene is isolated and characterized from tomato (Solanum lycopersicum L.). The SlCRK1 has two C-X8-C-X2-C motifs: a trans-membrane region and a kinase domain similar to other CRKs. The semi-quantitative RT-PCR exhibits the specific expression of SlCRK1 in the flower, but not in the root, leaf, seed, and fruit of the tomato. In addition, SlCRK1 exhibits pollen-specific expression in the floral organ. SlCRK1 has pollen-specific cis-acting elements in the promoter region, and its promoter has pollen-specific activity in the homozygous transgenic plants of tomato and Arabidopsis as confirmed through histochemical GUS assays. Moreover, the expression of SlCRK1 is not detected via stress treatment or hormone treatment. In this study, SlCRK1 from tomato is characterized and its promoter can be useful in developing transgenic plants with foreign genes that should be expressed in pollens.

Keywords

TomatoArabidopsisSlCRK1PromoterPollenTissue-specific gene

Supplementary material

13258_2013_168_MOESM1_ESM.pdf (128 kb)
Supplementary Fig. 1 Expression level of SlCRK1 during stress conditions and hormone treatments in tomato plants. Transcription levels of the SlCRK1 gene at various time intervals after the abiotic stress treatments, exogenous application of plant hormones, pathogen treatment (DC3000), and various growth stages (hour: h, week: w)
13258_2013_168_MOESM2_ESM.pdf (49 kb)
Supplementary Fig. 2 Histochemical GUS assay of the wild-type tomato and Arabidopsis. The tomato flower and pollen from a nine-week-old plant and four-week-old Arabidopsis plants are presented. The scale bars in flower are 1 mm and the bars in pollen are 50 μm

Copyright information

© The Genetics Society of Korea 2013

Authors and Affiliations

  • Woong Bom Kim
    • 1
    • 2
  • So Young Yi
    • 1
  • Sang-Keun Oh
    • 3
  • Chan Ju Lim
    • 1
  • Hyun A. Kim
    • 1
    • 4
  • Hyun A. Jang
    • 1
    • 2
  • Ha Yeon Lee
    • 1
  • Youn-Il Park
    • 2
  • Suk-Yoon Kwon
    • 1
    • 4
  1. 1.Plant Systems Engineering Research CenterKorea Research Institute of Bioscience and BiotechnologyDaejonRepublic of Korea
  2. 2.Department of Biology, School of Bioscience & BiotechnologyChungnam National UniversityDaejonRepublic of Korea
  3. 3.Plant Genomics and Breeding InstituteSeoul National UniversitySeoulRepublic of Korea
  4. 4.Biosystems and Bioengineering ProgramUniversity of Science and TechnologyDaejonRepublic of Korea