Protein & Cell

, Volume 5, Issue 11, pp 805-815

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Applications of RNA interference high-throughput screening technology in cancer biology and virology

  • Shan GaoAffiliated withDepartment of Oncology, John Radcliffe Hospital, Weatherall Institute of Molecular Medicine, University of Oxford Email author 
  • , Chen YangAffiliated withDepartment of Molecular Genetics and Microbiology, Stony Brook University Email author 
  • , Shan JiangAffiliated withDepartment of Immunology, Duke University
  • , Xiao-Ning XuAffiliated withDepartment of Immunology, Chelsea & Westminster Hospital, Imperial College of London
  • , Xin LuAffiliated withNuffield Department of Clinical Medicine, Ludwig Institute for Cancer Research Oxford Branch, University of Oxford
  • , You-Wen HeAffiliated withDepartment of Immunology, Duke University
  • , Annie CheungAffiliated withDepartment of Pathology, Queen Mary Hospital, University of Hong Kong
  • , Hui WangAffiliated withNatural Environment Research Council, Centre for Ecology & HydrologyDepartment of Zoology, University of Oxford Email author 


RNA interference (RNAi) is an ancient intra-cellular mechanism that regulates gene expression and cell function. Large-scale gene silencing using RNAi high-throughput screening (HTS) has opened an exciting frontier to systematically study gene function in mammalian cells. This approach enables researchers to identify gene function in a given biological context and will provide considerable novel insight. Here, we review RNAi HTS strategies and applications using case studies in cancer biology and virology.


RNA interference (RNAi) short interfering RNA (siRNA) short hairpin RNA (shRNA) high-throughput screening cancer virology