Protein & Cell

pp 1–10

Global DNA methylation and transcriptional analyses of human ESC-derived cardiomyocytes

  • Ying Gu
  • Guang-Hui Liu
  • Nongluk Plongthongkum
  • Christopher Benner
  • Fei Yi
  • Jing Qu
  • Keiichiro Suzuki
  • Jiping Yang
  • Weiqi Zhang
  • Mo Li
  • Nuria Montserrat
  • Isaac Crespo
  • Antonio del Sol
  • Concepcion Rodriguez Esteban
  • Kun Zhang
  • Juan Carlos Izpisua Belmonte
Research article Protein & Cell

DOI: 10.1007/s13238-013-3911-2

Cite this article as:
Gu, Y., Liu, GH., Plongthongkum, N. et al. Protein Cell (2013). doi:10.1007/s13238-013-3911-2

Abstract

With defined culture protocol, human embryonic stem cells (hESCs) are able to generate cardiomyocytes in vitro, therefore providing a great model for human heart development, and holding great potential for cardiac disease therapies. In this study, we successfully generated a highly pure population of human cardiomyocytes (hCMs) (>95% cTnT+) from hESC line, which enabled us to identify and characterize an hCM-specific signature, at both the gene expression and DNA methylation levels. Gene functional association network and gene-disease network analyses of these hCM-enriched genes provide new insights into the mechanisms of hCM transcriptional regulation, and stand as an informative and rich resource for investigating cardiac gene functions and disease mechanisms. Moreover, we show that cardiac-structural genes and cardiac-transcription factors have distinct epigenetic mechanisms to regulate their gene expression, providing a better understanding of how the epigenetic machinery coordinates to regulate gene expression in different cell types.

Keywords

human cardiomyocyte DNA methylation microarray heart development 

Supplementary material

13238_2013_3911_MOESM1_ESM.zip (29.1 mb)
Supplementary material, approximately 29.1 MB.

Copyright information

© Higher Education Press and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Ying Gu
    • 1
  • Guang-Hui Liu
    • 2
    • 3
  • Nongluk Plongthongkum
    • 4
  • Christopher Benner
    • 1
  • Fei Yi
    • 1
  • Jing Qu
    • 2
  • Keiichiro Suzuki
    • 1
  • Jiping Yang
    • 2
  • Weiqi Zhang
    • 2
  • Mo Li
    • 1
  • Nuria Montserrat
    • 5
    • 6
  • Isaac Crespo
    • 7
  • Antonio del Sol
    • 7
  • Concepcion Rodriguez Esteban
    • 1
  • Kun Zhang
    • 4
  • Juan Carlos Izpisua Belmonte
    • 1
    • 5
  1. 1.Gene Expression LaboratorySalk Institute for Biological StudiesLa JollaUSA
  2. 2.National Laboratory of Biomacromolecules, Institute of BiophysicsChinese Academy of SciencesBeijingChina
  3. 3.State key Laboratory of Drug Research, Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghaiChina
  4. 4.Department of BioengineeringUniversity of California at San DiegoLa JollaUSA
  5. 5.Center of Regenerative Medicine in BarcelonaBarcelonaSpain
  6. 6.Biomedical Research Networking center in BioengineeringBiomaterials and Nanomedicine (CIBER-BBN)ZaragozaSpain
  7. 7.Luxembourg Centre for Systems Biomedicine (LCSB)University of LuxembourgLuxembourg CityLuxembourg

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