Protein & Cell

, Volume 3, Issue 12, pp 934–942

Compound screening platform using human induced pluripotent stem cells to identify small molecules that promote chondrogenesis

Authors

  • Sheng-Lian Yang
    • Gene Expression LaboratoriesThe Salk Institute for Biological Studies
  • Erica Harnish
    • Sanofi US, R&D, Early to Candidate UnitTucson Research Center
  • Thomas Leeuw
    • Sanofi Deutschland GmbH, R&DTSU Aging Quality of Life, Industriepark Hoechst
  • Uwe Dietz
    • Sanofi Deutschland GmbH, R&DTSU Aging Quality of Life, Industriepark Hoechst
  • Erika Batchelder
    • Gene Expression LaboratoriesThe Salk Institute for Biological Studies
  • Paul S. Wright
    • Sanofi US, R&D, Early to Candidate UnitTucson Research Center
  • Jane Peppard
    • Sanofi US, R&D, Early to Candidate UnitTucson Research Center
  • Paul August
    • Sanofi US, R&D, Early to Candidate UnitTucson Research Center
  • Cecile Volle-Challier
    • Sanofi R&DEarly to Candidate Unit
  • Francoise Bono
    • Sanofi R&DEarly to Candidate Unit
  • Jean-Marc Herbert
    • Sanofi R&DEarly to Candidate Unit
    • Gene Expression LaboratoriesThe Salk Institute for Biological Studies
    • Center of Regeneration Medicine
Communication

DOI: 10.1007/s13238-012-2107-5

Cite this article as:
Yang, S., Harnish, E., Leeuw, T. et al. Protein Cell (2012) 3: 934. doi:10.1007/s13238-012-2107-5

Abstract

Articular cartilage, which is mainly composed of collagen II, enables smooth skeletal movement. Degeneration of collagen II can be caused by various events, such as injury, but degeneration especially increases over the course of normal aging. Unfortunately, the body does not fully repair itself from this type of degeneration, resulting in impaired movement. Microfracture, an articular cartilage repair surgical technique, has been commonly used in the clinic to induce the repair of tissue at damage sites. Mesenchymal stem cells (MSC) have also been used as cell therapy to repair degenerated cartilage. However, the therapeutic outcomes of all these techniques vary in different patients depending on their age, health, lesion size and the extent of damage to the cartilage. The repairing tissues either form fibrocartilage or go into a hypertrophic stage, both of which do not reproduce the equivalent functionality of endogenous hyaline cartilage. One of the reasons for this is inefficient chondrogenesis by endogenous and exogenous MSC. Drugs that promote chondrogenesis could be used to induce self-repair of damaged cartilage as a non-invasive approach alone, or combined with other techniques to greatly assist the therapeutic outcomes. The recent development of human induced pluripotent stem cell (iPSCs), which are able to self-renew and differentiate into multiple cell types, provides a potentially valuable cell resource for drug screening in a “more relevant” cell type. Here we report a screening platform using human iPSCs in a multi-well plate format to identify compounds that could promote chondrogenesis.

Keywords

hESChiPSCchondrogenesiscompound screening platform

Copyright information

© Higher Education Press and Springer-Verlag Berlin Heidelberg 2012