Protein & Cell

, Volume 2, Issue 10, pp 845–854

SDF-1/CXCR4 axis modulates bone marrow mesenchymal stem cell apoptosis, migration and cytokine secretion

Authors

  • Xiaolei Liu
    • The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life ScienceNankai University
  • Biyan Duan
    • College of PharmacyNankai University
  • Zhaokang Cheng
    • The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life ScienceNankai University
  • Xiaohua Jia
    • The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life ScienceNankai University
  • Lina Mao
    • The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life ScienceNankai University
    • Oklahoma Medical Research Foundation
  • Hao Fu
    • The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life ScienceNankai University
  • Yongzhe Che
    • School of MedicineNankai University
    • The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life ScienceNankai University
  • Lin Liu
    • The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life ScienceNankai University
    • The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life ScienceNankai University
Research Article

DOI: 10.1007/s13238-011-1097-z

Cite this article as:
Liu, X., Duan, B., Cheng, Z. et al. Protein Cell (2011) 2: 845. doi:10.1007/s13238-011-1097-z

Abstract

Bone marrow mesenchymal stem cells (MSCs) are considered as a promising cell source to treat the acute myocardial infarction. However, over 90% of the stem cells usually die in the first three days of transplantation. Survival potential, migration ability and paracrine capacity have been considered as the most important three factors for cell transplantation in the ischemic cardiac treatment. We hypothesized that stromal-derived factor-1 (SDF-1)/CXCR4 axis plays a critical role in the regulation of these processes. In this study, apoptosis was induced by exposure of MSCs to H2O2 for 2 h. After re-oxygenation, the SDF-1 pretreated MSCs demonstrated a significant increase in survival and proliferation. SDF-1 pretreatment also enhanced the migration and increased the secretion of pro-survival and angiogenic cytokines including basic fibroblast growth factor and vascular endothelial growth factor. Western blot and RT-PCR demonstrated that SDF-1 pretreatment significantly activated the pro-survival Akt and Erk signaling pathways and up-regulated Bcl-2/Bax ratio. These protective effects were partially inhibited by AMD3100, an antagonist of CXCR4.We conclude that the SDF-1/CXCR4 axis is critical for MSC survival, migration and cytokine secretion.

Keywords

SDF-1/CXCR4bone marrow mesenchymal stem cellssurvivalmigrationsecretion

Copyright information

© Higher Education Press and Springer-Verlag Berlin Heidelberg 2011