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Phylogeny, identification and pathogenicity of the Botryosphaeriaceae associated with collar and root rot of the biofuel plant Jatropha curcas in Brazil, with a description of new species of Lasiodiplodia

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Abstract

The global expansion of cultivation areas of Jatropha has contributed to the emergence of various diseases. Currently in Brazil, the occurrence of a new disease has been reported that not only reduces the productivity but also causes the death of Jatropha. This disease is associated with collar and root rot of plants. From morphological and phylogenetic studies (based on Internal Transcribed Spacers, β-tubulin and Translation Elongation Factor 1-α sequences), nine species of Botryosphaeriaceae were identified. These species include Lasiodiplodia egyptiacae, L. pseudotheobromae, L. theobromae, Macrophomina phaseolina, Neoscytalidium hyalinum and four Lasiodiplodia spp. that are proposed as new species (L. euphorbicola, L. jatrophicola, L.macrospora and L. subglobosa). All the species in this study, except M. phaseolina, are pathogenic. The results show that root rot of physic nut plants is caused by complex pathogens. This study provides new information for future studies of disease management, quarantine programs and, especially, the development of resistant varieties for collar and root rot disease in J. curcas.

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Acknowledgments

This work forms part of a research project submitted as an M.Sc. dissertation to the Departamento de Fitopatologia/Universidade Federal de Viçosa by A. R. Machado. The authors thank Fundação de Amparo a Pesquisa do Estado de Minas Gerais –FAPEMIG for the financial support of the work (CAG-APQ-00987-11) and Conselho Nacional de Desenvolvimento Científico e Tecnológico –CNPq for authors fellowships.

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  1. Departamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa, 36570-000, Brazil

    Alexandre Reis Machado, Danilo Batista Pinho & Olinto Liparini Pereira

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  1. Alexandre Reis Machado
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  2. Danilo Batista Pinho
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Correspondence to Olinto Liparini Pereira.

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Machado, A.R., Pinho, D.B. & Pereira, O.L. Phylogeny, identification and pathogenicity of the Botryosphaeriaceae associated with collar and root rot of the biofuel plant Jatropha curcas in Brazil, with a description of new species of Lasiodiplodia . Fungal Diversity 67, 231–247 (2014). https://doi.org/10.1007/s13225-013-0274-1

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