Fungal Diversity

, Volume 49, Issue 1, pp 113–123

Assessment of soil fungal diversity in different alpine tundra habitats by means of pyrosequencing

Authors

  • Guillaume Lentendu
    • Laboratoire d’Ecologie Alpine, CNRS UMR 5553Université Joseph Fourier, Grenoble 1
    • Department Soil EcologyUFZ—Helmholtz Centre for Environmental Research
  • Lucie Zinger
    • Laboratoire d’Ecologie Alpine, CNRS UMR 5553Université Joseph Fourier, Grenoble 1
    • Max Plank Institute for Marine Microbiology
  • Stéphanie Manel
    • Laboratoire d’Ecologie Alpine, CNRS UMR 5553Université Joseph Fourier, Grenoble 1
    • Laboratoire Population Environnement Développement, UMR 151 UP/IRDUniversité de Provence
    • Laboratoire d’Ecologie Alpine, CNRS UMR 5553Université de Grenoble
  • Eric Coissac
    • Laboratoire d’Ecologie Alpine, CNRS UMR 5553Université Joseph Fourier, Grenoble 1
  • Philippe Choler
    • Laboratoire d’Ecologie Alpine, CNRS UMR 5553Université Joseph Fourier, Grenoble 1
    • Station Alpine J. FourierGrenoble Univ, CNRS UMS 2925
    • Laboratoire d’Ecologie Alpine, CNRS UMR 5553Université Joseph Fourier, Grenoble 1
  • Christelle Melodelima
    • Laboratoire d’Ecologie Alpine, CNRS UMR 5553Université Joseph Fourier, Grenoble 1
Article

DOI: 10.1007/s13225-011-0101-5

Cite this article as:
Lentendu, G., Zinger, L., Manel, S. et al. Fungal Diversity (2011) 49: 113. doi:10.1007/s13225-011-0101-5

Abstract

Studying fungal diversity is vital if we want to shed light on terrestrial ecosystem functioning. However, there is still poor understanding of fungal diversity and variation given that Fungi are highly diversified and that most of fungal species remain uncultured. In this study we explored diversity with 454 FLX sequencing technology by using the Internal Transcribed Spacer 1 (ITS1) as the fungal barcode marker in order to evaluate the effect of 11 environmental conditions on alpine soil fungal diversity, as well as the consistency of those results by taking into account rare or unidentified Molecular Operational Taxonomic Units (MOTUs). In total we obtained 205131 ITS1 reads corresponding to an estimated fungal gamma diversity of between 5100 and 12 000 MOTUs at a 98% similarity threshold when considering respectively only identified fungal and all MOTUs. Fungal beta-diversity patterns were significantly explained by the environmental conditions, and were very consistent for abundant/rare and fungal/unidentified MOTUs confirming the ecological significance of rare/unidentified MOTUs, and therefore the existence of a fungal rare biosphere. This study shows that a beta-diversity estimation based on pyrosequencing is robust enough to support ecological studies. Additionally, our results suggest that rare MOTUs harbour ecological information. Thus the fungal rare biosphere may be important for ecosystem dynamics and resilience.

Keywords

Multiple-tag parallel pyrosequencingFungal communitiesMOTUsRare biosphereAlpine tundraLandscape

Supplementary material

13225_2011_101_MOESM1_ESM.pdf (17 kb)
Online resource 1(PDF 17.3 kb)

Copyright information

© Kevin D. Hyde 2011