Annals of Microbiology

, Volume 63, Issue 3, pp 1083–1091

Interaction between the entomopathogenic bacterium Bacillus thuringiensis subsp. kurstaki and two entomopathogenic fungi in bio-control of Sesamia nonagrioides (Lefebvre) (Lepidoptera: Noctuidae)

  • Spiridon Mantzoukas
  • Panagiotis Milonas
  • Dimitris Kontodimas
  • Kostas Angelopoulos
Original Article

DOI: 10.1007/s13213-012-0565-x

Cite this article as:
Mantzoukas, S., Milonas, P., Kontodimas, D. et al. Ann Microbiol (2013) 63: 1083. doi:10.1007/s13213-012-0565-x

Abstract

The interactions between the entomopathogenic bacterium Bacillus thuringiensis ssp. kurstaki and two entomopathogenic fungi Beauveria bassiana Balsamo (Vuillemin) (Hypocreales: Cordycipitaceae) and Metarhizium robertsii (Metchnikoff) Sorokin (Hypocreales: Clavicipitaceae) were examined on larvae of Sesamia nonagrioides (Lefebvre) (Lepidoptera: Noctuidae) in 8, 13 and 16 days post-treatment intervals. An overall positive interaction between the pathogens was observed and the larval mortality at 16 days was 56–100 % exposed to M. robertsii combined with B. thuringiensis subsp. kurstaki, whereas B. bassiana combined with B. thuringiensis ssp. kurstaki killed 54–100 % of exposed larvae. After 8 days, in 6 of the combinations, we found an additive relationship between the pathogens, whereas, a negative interaction was observed in 10 of them. In contrast, after 13 days, in 2 of the combinations the positive interaction could be considered as synergistic between pathogens, in 10 as additive, and in only 4 as negative. Finally, after 16 days, in 11 of the combinations we found an additive connection between the pathogens, wheras a negative interaction was seen in 5. Applying both pathogens simultaneously offers a method of Sesamia nonagrioides control that could be more effective than using each pathogen separately.

Keywords

Pathogen interactionBeauveria bassianaMetarhizium robetsiiBacillus thuringiensis subsp. kurstakiSesamia nonagrioides

Copyright information

© Springer-Verlag Berlin Heidelberg and the University of Milan 2012

Authors and Affiliations

  • Spiridon Mantzoukas
    • 1
  • Panagiotis Milonas
    • 2
  • Dimitris Kontodimas
    • 2
  • Kostas Angelopoulos
    • 1
  1. 1.Laboratory of Plant Physiology, Department of BiologyUniversity of PatrasPatrasGreece
  2. 2.Department of Entomology and Agricultural ZoologyBenaki Phytopathological InstituteAthensGreece