Annals of Microbiology

, Volume 60, Issue 1, pp 75–80

Investigations into the antibacterial behavior of copper nanoparticles against Escherichia coli

  • Muhammad Raffi
  • Saba Mehrwan
  • Tariq Mahmood Bhatti
  • Javed Iqbal Akhter
  • Abdul Hameed
  • Wasim Yawar
  • M. Masood ul Hasan
Original Article

DOI: 10.1007/s13213-010-0015-6

Cite this article as:
Raffi, M., Mehrwan, S., Bhatti, T.M. et al. Ann Microbiol (2010) 60: 75. doi:10.1007/s13213-010-0015-6
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Abstract

Zerovalent copper nanoparticles (Cu0) of 12 nm size were synthesized using an inert gas condensation method in which bulk copper metal was evaporated into an inert environment of argon with subsequent cooling for nucleation and growth of nanoparticles. Crystalline structure, morphology and estimation of size of nanoparticles were carried out by X-ray diffraction and transmission electron microscopy. The antibacterial activity of these nanoparticles against the Gram-negative bacterium Escherichia coli was assessed in liquid as well as solid growth media. It was observed from scanning electron microscopic analysis that the interaction of copper nanoparticles with E. coli resulted in the formation of cavities/pits in the bacterial cell wall. The antibacterial property of copper nanoparticles was attributed mainly to adhesion with bacteria because of their opposite electrical charges, resulting in a reduction reaction at the bacterial cell wall. Nanoparticles with a larger surface-to-volume ratio provide more efficient means for antibacterial activity.

Keywords

Copper nanoparticleAntibacterial activityEscherichia coliX-ray diffractionElectron microscopy

Copyright information

© Springer-Verlag and the University of Milan 2010

Authors and Affiliations

  • Muhammad Raffi
    • 1
  • Saba Mehrwan
    • 2
  • Tariq Mahmood Bhatti
    • 3
  • Javed Iqbal Akhter
    • 4
  • Abdul Hameed
    • 2
  • Wasim Yawar
    • 3
  • M. Masood ul Hasan
    • 1
  1. 1.Department of Chemical and Materials EngineeringPakistan Institute of Engineering and Applied Sciences (PIEAS)IslamabadPakistan
  2. 2.Department of Microbiology, Faculty of Biological SciencesQuaid-i-Azam UniversityIslamabadPakistan
  3. 3.Central Analytical Facility DivisionPakistan Institute of Nuclear Science and Technology (PINSTECH)IslamabadPakistan
  4. 4.Physics DivisionPakistan Institute of Nuclear Science and Technology (PINSTECH)IslamabadPakistan