Organisms Diversity & Evolution

, Volume 14, Issue 4, pp 369–382

Molecular identification and morphological characteristics of native and invasive Asian brush-clawed crabs (Crustacea: Brachyura) from Japanese and German coasts: Hemigrapsus penicillatus (De Haan, 1835) versus Hemigrapsus takanoi Asakura & Watanabe 2005

  • Alexandra Markert
  • Michael J. Raupach
  • Alexandra Segelken-Voigt
  • Achim Wehrmann
Original Article

DOI: 10.1007/s13127-014-0176-4

Cite this article as:
Markert, A., Raupach, M.J., Segelken-Voigt, A. et al. Org Divers Evol (2014) 14: 369. doi:10.1007/s13127-014-0176-4

Abstract

Since 1994, Hemigrapsus penicillatus, an Asian brush-clawed shore crab, spreads along Northeast Atlantic coasts. There is evidence that the most recently described and widely accepted sibling species of H. penicillatus, namely Hemigrapsus takanoi, represents the Asian brush-clawed brachyuran crab in Europe. Morphological characteristics are considered insufficient for species discrimination, and molecular analyses were recommended but have to date not been performed in Europe. To clarify the identity of the non-indigenous Asian brush-clawed crab species, which has been invading intertidal alien Crassostrea reefs in the Wadden Sea, we analysed more than 3.5 kpb of mitochondrial and nuclear genes of individuals from invaded German sites and from native Japanese sites. In addition to molecular analyses, we also document key morphological and morphometric characteristics of the same individuals to provide a comparative description. While morphological identification was not confidently feasible, our molecular results confirm the existence of Hemigrapsus takanoi as a closely related species to H. penicillatus and have identified H. takanoi to be the alien brush-clawed crab species in Germany. Furthermore, most of the analysed specimens from Japan and all additional NCBI-listed brush-clawed crabs from Japan, Korea and China which were traditionally classified as H. penicillatus in Asia, are de facto H. takanoi.

Keywords

Sibling species DNA barcoding 16S rDNA NaK-ATPase Bioinvasion North Sea 

Supplementary material

13127_2014_176_MOESM1_ESM.pdf (29 kb)
Online Resource 1(PDF 29 kb)
13127_2014_176_MOESM2_ESM.pdf (30 kb)
Online Resource 2(PDF 29 kb)
13127_2014_176_MOESM3_ESM.pdf (16 kb)
Online Resource 3(PDF 15 kb)

Copyright information

© Gesellschaft für Biologische Systematik 2014

Authors and Affiliations

  • Alexandra Markert
    • 1
    • 3
  • Michael J. Raupach
    • 2
  • Alexandra Segelken-Voigt
    • 1
    • 4
  • Achim Wehrmann
    • 1
    • 3
  1. 1.Marine Research DepartmentSenckenberg am MeerWilhelmshavenGermany
  2. 2.German Centre for Marine BiodiversitySenckenberg am MeerWilhelmshavenGermany
  3. 3.Biodiversity and Climate Research Centre (BiK-F)FrankfurtGermany
  4. 4.Department of Biology and Environmental ScienceUniversity of OldenburgOldenburgGermany