Translational Stroke Research

, Volume 3, Issue 4, pp 500–507

GPER1/GPR30 Activation Improves Neuronal Survival Following Global Cerebral Ischemia Induced by Cardiac Arrest in Mice

  • Y. Kosaka
  • N. Quillinan
  • C. T. Bond
  • R. J. Traystman
  • P. D. Hurn
  • P. S. Herson
Original Article

DOI: 10.1007/s12975-012-0211-8

Cite this article as:
Kosaka, Y., Quillinan, N., Bond, C.T. et al. Transl. Stroke Res. (2012) 3: 500. doi:10.1007/s12975-012-0211-8

Abstract

Female sex steroids, particularly estrogens, contribute to the sexually dimorphic response observed in cerebral ischemic outcome, with females being relatively protected compared to males. Using a mouse model of cardiac arrest and cardiopulmonary resuscitation, we previously demonstrated that estrogen neuroprotection is mediated in part by the estrogen receptor β, with no involvement of estrogen receptor α. In this study, we examined the neuroprotective effect of the novel estrogen receptor, G protein-coupled estrogen receptor 1 (GPER1/GPR30). Male mice administered with the GPR30 agonist G1 exhibited significantly reduced neuronal injury in the hippocampal CA1 region and striatum. The magnitude of neuroprotection observed in G1-treated mice was indistinguishable from estrogen-treated mice, implicating GPR30 in estrogen neuroprotection. Real-time quantitative RT-PCR indicates that G1 treatment increases expression of the neuroprotective ion channel, small-conductance calcium-activated potassium channel 2. We conclude that GPR30 agonists show promise in reducing brain injury following global cerebral ischemia.

Keywords

Cardiac arrestGPR30/GPER1SK2Cerebral ischemiaG1

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Y. Kosaka
    • 1
  • N. Quillinan
    • 1
    • 2
  • C. T. Bond
    • 3
  • R. J. Traystman
    • 2
    • 4
  • P. D. Hurn
    • 5
  • P. S. Herson
    • 1
    • 2
  1. 1.Department of Anesthesiology and Perioperative MedicineOregon Health and Science UniversityPortlandUSA
  2. 2.Department of AnesthesiologyUniversity of Colorado DenverAuroraUSA
  3. 3.Vollum InstituteOregon Health and Science UniversityPortlandUSA
  4. 4.Department of PharmacologyUniversity of Colorado DenverAuroraUSA
  5. 5.Department of NeurobiologyUniversity of Texas at AustinAustinUSA