Original Article

Translational Stroke Research

, Volume 3, Supplement 1, pp 174-179

First online:

Micro-Computed Tomography for Hemorrhage Disruption of Mouse Brain Vasculature

  • Bohua XieAffiliated withSchool of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong UniversitySchool of Life Science and Biotechnology, Shanghai Jiao Tong University
  • , Peng MiaoAffiliated withSchool of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong UniversitySchool of Communication and Information Engineering, Shanghai University
  • , Yuhao SunAffiliated withSchool of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong UniversityDepartment of Neurosurgery, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University
  • , Yongting WangAffiliated withSchool of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University
  • , Guo-Yuan YangAffiliated withSchool of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong UniversityDepartment of Neurology, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong UniversityNeuroscience and Neuroengineering Center, Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University Email author 

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Abstract

The use of genetic engineering to develop important neuropathological mouse models has made cerebrovascular imaging essential for the investigation of numerous brain disorders, especially cerebrovascular disorders, such as aneurysms, arteriovenous malformations, and ischemic and hemorrhagic stroke. New laboratory-based X-ray microimagers exist that provide easy access, reliable operation, and performance previously found only in synchrotron-based instruments. Here, we reported a novel approach using such a system to detect intracerebral hemorrhage and resultant cerebrovascular pathology. Adult male C57BL/6 mice (n = 12) underwent 30 μl autologous blood injection into the right basal ganglia region. After sacrificing the animals and vascular perfusion with Microfil® MV-122 Yellow to opacify vascular and microvascular structures, the brain was post-fixed and partially hydrated for 3D imaging with a MicroXCT-400® at 30 KeV and 2-μm resolution. Tomographic reconstruction of high-resolution microimages was accomplished with Amira® software. High-quality 3D images included cerebrocortical microvessels, the circle of Willis, the sagittal sinus, transverse sinus, and other arterial and venous systems. In the ipsilateral hemisphere, there clearly were early-stage vasodilatation and later-stage neovascularization. Very high-resolution, laboratory-based, X-ray micro-CT contrast imaging can accomplish sensitive quantifications of normal and pathological small cerebrovascular changes, especially in hemorrhagic stroke and subsequent hemorrhage-induced neovascularization.

Keywords

Brain Hemorrhage Imaging Micro-XCT Mouse Vasculature