Translational Stroke Research

, Volume 3, Supplement 1, pp 174–179

Micro-Computed Tomography for Hemorrhage Disruption of Mouse Brain Vasculature

Authors

  • Bohua Xie
    • School of Biomedical Engineering and Med-X Research InstituteShanghai Jiao Tong University
    • School of Life Science and BiotechnologyShanghai Jiao Tong University
  • Peng Miao
    • School of Biomedical Engineering and Med-X Research InstituteShanghai Jiao Tong University
    • School of Communication and Information EngineeringShanghai University
  • Yuhao Sun
    • School of Biomedical Engineering and Med-X Research InstituteShanghai Jiao Tong University
    • Department of Neurosurgery, Ruijin Hospital, School of MedicineShanghai Jiao Tong University
  • Yongting Wang
    • School of Biomedical Engineering and Med-X Research InstituteShanghai Jiao Tong University
    • School of Biomedical Engineering and Med-X Research InstituteShanghai Jiao Tong University
    • Department of Neurology, Ruijin Hospital, School of MedicineShanghai Jiao Tong University
    • Neuroscience and Neuroengineering Center, Med-X Research Institute and School of Biomedical EngineeringShanghai Jiao Tong University
Original Article

DOI: 10.1007/s12975-012-0164-y

Cite this article as:
Xie, B., Miao, P., Sun, Y. et al. Transl. Stroke Res. (2012) 3: 174. doi:10.1007/s12975-012-0164-y
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Abstract

The use of genetic engineering to develop important neuropathological mouse models has made cerebrovascular imaging essential for the investigation of numerous brain disorders, especially cerebrovascular disorders, such as aneurysms, arteriovenous malformations, and ischemic and hemorrhagic stroke. New laboratory-based X-ray microimagers exist that provide easy access, reliable operation, and performance previously found only in synchrotron-based instruments. Here, we reported a novel approach using such a system to detect intracerebral hemorrhage and resultant cerebrovascular pathology. Adult male C57BL/6 mice (n = 12) underwent 30 μl autologous blood injection into the right basal ganglia region. After sacrificing the animals and vascular perfusion with Microfil® MV-122 Yellow to opacify vascular and microvascular structures, the brain was post-fixed and partially hydrated for 3D imaging with a MicroXCT-400® at 30 KeV and 2-μm resolution. Tomographic reconstruction of high-resolution microimages was accomplished with Amira® software. High-quality 3D images included cerebrocortical microvessels, the circle of Willis, the sagittal sinus, transverse sinus, and other arterial and venous systems. In the ipsilateral hemisphere, there clearly were early-stage vasodilatation and later-stage neovascularization. Very high-resolution, laboratory-based, X-ray micro-CT contrast imaging can accomplish sensitive quantifications of normal and pathological small cerebrovascular changes, especially in hemorrhagic stroke and subsequent hemorrhage-induced neovascularization.

Keywords

BrainHemorrhageImagingMicro-XCTMouseVasculature

Abbreviations

ACA

Anterior cerebral artery

BA

Basal artery

ICA

Internal carotid artery

ICH

Intracerebral hemorrhage

MCA

Middle cerebral artery

Micro-XCT

Micro-X-ray computerized tomography

3D

Three dimensional

VOI

Volume of interest

Copyright information

© Springer Science+Business Media, LLC 2012