Development of nuclear SNP markers for genetic tracking of Iroko, Milicia excelsa and Milicia regia

  • C. Blanc-Jolivet
  • B. Kersten
  • K. Daïnou
  • O. Hardy
  • E. Guichoux
  • A. Delcamp
  • B. Degen
Technical Note

DOI: 10.1007/s12686-017-0716-2

Cite this article as:
Blanc-Jolivet, C., Kersten, B., Daïnou, K. et al. Conservation Genet Resour (2017). doi:10.1007/s12686-017-0716-2
  • 30 Downloads

Abstract

Restriction associated DNA sequencing was conducted on two genetically independent individuals of Iroko, Milicia excelsa, for the discovery of nuclear SNPs. Ninety-four samples, well-distributed over the natural range and including timber DNA, were screened at 138 loci on a MassARRAY iPLEX system. Amplification success was high and 77 loci were selected to design a set of markers for genetic timber tracking purposes.

Keywords

Single nucleotide polymorphism Milicia RAD sequencing MassARRAY Timber tracking 

Supplementary material

12686_2017_716_MOESM1_ESM.docx (83 kb)
Supplementary material 1 (DOCX 83 KB)

Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • C. Blanc-Jolivet
    • 1
  • B. Kersten
    • 1
  • K. Daïnou
    • 2
    • 3
  • O. Hardy
    • 4
  • E. Guichoux
    • 5
  • A. Delcamp
    • 5
  • B. Degen
    • 1
  1. 1.Thünen Institute of Forest GeneticsGrosshansdorfGermany
  2. 2.Nature+ asbl s/c BIOSE, Management of Forest Resources, GxABT, ULgGemblouxBelgium
  3. 3.Université Nationale d’Agriculture de Porto NovoPorto NovoBenin
  4. 4.Faculté des Sciences, Evolutionary Biology and EcologyUniversité Libre de BruxellesBrusselsBelgium
  5. 5.BIOGECO, INRA, University of BordeauxCestasFrance

Personalised recommendations