Conservation Genetics Resources

, Volume 5, Issue 3, pp 697–701

Isolation and characterization of eighteen microsatellite loci for the largemouth bass, Micropterus salmoides, and cross amplification in congeneric species

  • Seifu Seyoum
  • Brandon L. Barthel
  • Michael D. Tringali
  • Michelle C. Davis
  • Samantha L. Schmitt
  • Pam S. Bellotti
  • Wesley F. Porak
Technical Note

DOI: 10.1007/s12686-013-9885-9

Cite this article as:
Seyoum, S., Barthel, B.L., Tringali, M.D. et al. Conservation Genet Resour (2013) 5: 697. doi:10.1007/s12686-013-9885-9

Abstract

We used a PCR-based technique to isolate 18 microsatellite loci for the largemouth bass, Micropterus salmoides. These loci were characterized using sampled populations in Florida and Wisconsin. The mean number of alleles at a locus was 8 for the sample from Florida and 5 for the samples from Wisconsin. The observed heterozygosities were 0.57 and 0.33 for the samples from Florida and Wisconsin, respectively. There was evidence of genetic differentiation between the Florida and Wisconsin samples at all 18 loci, indicating spatial structure within the range of M. salmoides. The markers cross-amplified in the seven other micropterid species, which suggests they would have utility for studies of hybridization among other members of the genus.

Keywords

Largemouth bass Micropterus salmoides Microsatellites Speciation 

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Seifu Seyoum
    • 1
  • Brandon L. Barthel
    • 1
  • Michael D. Tringali
    • 1
    • 2
  • Michelle C. Davis
    • 1
  • Samantha L. Schmitt
    • 1
  • Pam S. Bellotti
    • 1
  • Wesley F. Porak
    • 2
  1. 1.Florida Fish and Wildlife Research InstituteSaint PetersburgUSA
  2. 2.Florida Fish and Wildlife Research InstituteEustisUSA

Personalised recommendations