Abstract
Burrowing thalassinid shrimp are important ecosystem engineers in estuarine benthic communities. We characterized 31 microsatellite loci in two shrimp species, Neotrypaea californiensis and Upogebia pugettensis, which are of current management concern due to declining abundance and interactions with shellfish aquaculture. All loci were variable with three to 22 alleles per locus and observed heterozygosity of 0.22–1.00. There were only four deviations from Hardy–Weinberg expectations and no significant linkage disequilibrium. These are the first microsatellites characterized for these species and provide a useful tool for further characterization of population structure and diversity.
References
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Acknowledgments
This work was funded by USDA-ARS (CRIS Project Number 5358-31000-003-00D). We thank John Chapman, Lee McCoy, and Katelyn Bosley for assistance collecting animals and Brian Knaus for assistance with retrieving and processing Illumina data. We also thank Renee Bellinger and Katherine O’Malley for providing helpful comments on the manuscript.
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Dumbauld, B.R., Mercer, D.M. & Camara, M. Isolation and characterization of novel microsatellite loci in two species of burrowing shrimp, Neotrypaea californiensis and Upogebia pugettensis . Conservation Genet Resour 6, 353–356 (2014). https://doi.org/10.1007/s12686-013-0092-5
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DOI: https://doi.org/10.1007/s12686-013-0092-5