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Characterization of microsatellite loci markers for the critically endangered Montseny brook newt (Calotriton arnoldi)

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Abstract

Fifteen novel tetranucleotide polymorphic microsatellite loci are described and characterized for the critically endangered Montseny brook newt Calotriton arnoldi. Until now little is known about genetic variability and dispersal of this species across its very limited distribution area, mainly represented by two main sectors (eastern and western from the Tordera river valley). The level of genetic diversity of the new loci was assessed in 23 individuals from the eastern sector. Number of alleles per locus ranged from 2 to 8 (mean = 4.7) and observed and expected heterozygosity ranged from 0.087 to 1.000 and 0.162 to 0.814, respectively. Almost all loci were found to be in Hardy–Weinberg equilibrium and were not linked. These polymorphic loci can be used in population structure, gene flow, and population differentiation. The value of such information should be high for successful management and conservation of this potentially highly threatened species.

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Acknowledgments

We are grateful to all members of the CRFS Torreferrussa, and especially to M. Alonso, F. Carbonell, E. Obon and R. Larios. We also thank the DAAM department of the Generalitat de Catalunya and F. Amat. This research was supported by Miloca and Zoo de Barcelona (PRIC-2011).

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Authors and Affiliations

  1. Unitat de Zoologia, Facultat de Biociències, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès, Barcelona, Catalonia, Spain

    E. Valbuena-Ureña

  2. Centre de Fauna Salvatge de Torreferrussa, Catalan Wildlife Service—Forestal Catalana, Finca de Torreferrusa, Crta B-140, Km 4.5, 08130, Santa Perpètua de la Mogoda, Barcelona, Catalonia, Spain

    E. Valbuena-Ureña

  3. Department of Evolutionary Biology, Zoological Institute, Technische Universität Braunschweig, Mendelssohnstr. 4, 38106, Braunschweig, Germany

    S. Steinfartz

  4. Institute of Evolutionary Biology (CSIC-Universitat Pompeu Fabra), Passeig Marítim de la Barceloneta 37-49, 08003, Barcelona, Catalonia, Spain

    S. Carranza

Authors
  1. E. Valbuena-Ureña
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  2. S. Steinfartz
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  3. S. Carranza
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Correspondence to E. Valbuena-Ureña.

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Valbuena-Ureña, E., Steinfartz, S. & Carranza, S. Characterization of microsatellite loci markers for the critically endangered Montseny brook newt (Calotriton arnoldi). Conservation Genet Resour 6, 263–265 (2014). https://doi.org/10.1007/s12686-013-0082-7

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  • DOI: https://doi.org/10.1007/s12686-013-0082-7

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