Conservation Genetics Resources

, Volume 6, Issue 1, pp 223–227

Isolation and characterization of 15 microsatellite loci in the specialist butterfly Boloria eunomia

  • Delphine Legrand
  • Audrey Chaput-Bardy
  • Camille Turlure
  • Quentin Dubois
  • Michèle Huet
  • Nicolas Schtickzelle
  • Virginie M. Stevens
  • Michel Baguette
Microsatellite Letters

DOI: 10.1007/s12686-013-0067-6

Cite this article as:
Legrand, D., Chaput-Bardy, A., Turlure, C. et al. Conservation Genet Resour (2014) 6: 223. doi:10.1007/s12686-013-0067-6

Abstract

Boloria eunomia is a boreo-montane butterfly species suffering from habitat loss and isolation in the relictual part of its distribution range. Small populations persist in habitats scattered on plateaux or low mountains in western, central and southern Europe. Quantifying gene flow within and between these remnant populations is thus a crucial point to properly delineate metapopulations, to understand their dynamics and hence to design appropriate conservation plans for this butterfly species. We developed primers for the amplification of 15 microsatellites loci for B. eunomia. Thirteen loci were grouped in 2 multiplexes and amplified in 50 individuals from 5 populations to validate their use in population genetics. Gene diversity was on average 0.63 across populations. Null alleles and recurrent Hardy–Weinberg disequilibrium were detected in 7 loci. However, Fst estimates after correction for the presence of null alleles were highly correlated (0.91) to Fst estimates without correction. The loci developed here are thus usable for fine scale population genetic analyses and represent a very useful tool to quantify gene flow within and between metapopulations of B. eunomia.

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Delphine Legrand
    • 1
  • Audrey Chaput-Bardy
    • 2
  • Camille Turlure
    • 3
  • Quentin Dubois
    • 3
  • Michèle Huet
    • 1
  • Nicolas Schtickzelle
    • 3
  • Virginie M. Stevens
    • 1
  • Michel Baguette
    • 1
    • 4
  1. 1.CNRS USR 2936Station d’Ecologie Expérimentale du CNRSMoulisFrance
  2. 2.Groupe Ecologie et Conservation des VertébrésUniversité d’Angers, PRES UNAMAngers Cedex 01France
  3. 3.Biodiversity Research Centre, Earth and Life InstituteUniversité catholique de LouvainLouvain-la-NeuveBelgium
  4. 4.Evolution et Biodiversité, UMR 7205, Institut de SystématiqueMuséum National d’Histoire NaturelleParis Cedex 5France