, Volume 6, Issue 1, pp 185-187
Date: 27 Sep 2013

Isolation and characterization of fifteen microsatellite loci in Leopard grouper (Mycteroperca rosacea) via 454 pyrosequencing

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Abstract

Leopard grouper (Mycteroperca rosacea) are distributed from Bahía Magdalena, through the Gulf of California and as far south as Jalisco, Mexico. They are listed as Vulnerable on the IUCN Red List due to significant fishing-induced declines over the past decade. Microsatellite loci were isolated by constructing a shotgun genomic library and sequencing using 454 XL + titanium chemistry. We characterized 15 polymorphic loci in 120 samples from three geographic locations. Genotyping yielded 15–60 alleles per locus with observed levels of heterozygosity ranging from 0.575 to 0.975.