, Volume 4, Issue 3, pp 787-789
Date: 18 Apr 2012

Development and characterization of sixteen microsatellite markers for the federally endangered species: Leptodea leptodon (Bivalvia: Unionidae) using paired-end Illumina shotgun sequencing

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Abstract

We isolated and characterized a total of 16 microsatellite loci in Leptodea leptodon. Loci were screened in 24 individuals from across the species current range in Missouri. The number of alleles per locus ranged from 3 to 17, observed heterozygosity ranged from 0.208 to 1.000, and the probability of identity values ranged from 0.014 to 0.67. These new loci will be used for conducting investigations into the genetic structure and diversity of extant populations of this federally endangered species.