Conservation Genetics Resources

, Volume 4, Issue 3, pp 763–771

Isolation, characterization and predicted genome locations of ruff (Philomachus pugnax, AVES) microsatellite loci

  • Lindsay L. Farrell
  • Deborah A. Dawson
  • Gavin J. Horsburgh
  • Terry Burke
  • David B. Lank
Technical Note

DOI: 10.1007/s12686-012-9639-0

Cite this article as:
Farrell, L.L., Dawson, D.A., Horsburgh, G.J. et al. Conservation Genet Resour (2012) 4: 763. doi:10.1007/s12686-012-9639-0

Abstract

We identified 247 unique ruff (Philomachus pugnax) microsatellite sequences. Primer sets were designed from 102 selected loci and tested in 12–24 individuals from a captive population. Sequence homology was used to assign locations in the chicken (Gallus gallus) and/or zebra finch (Taeniopygia guttata) genome for the majority of these loci. Fifty-two loci were found to be polymorphic and 47 of these were typed in known families. Forty-six loci displayed Mendelian inheritance including Ppu058, which was confirmed to be Z-linked by the complete absence of any heterozygous females.

Keywords

LekMale morphsMicrosatelliteScolopacidaeShorebirdSimple tandem repeat (STR)

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Lindsay L. Farrell
    • 1
    • 2
  • Deborah A. Dawson
    • 1
  • Gavin J. Horsburgh
    • 1
  • Terry Burke
    • 1
  • David B. Lank
    • 2
  1. 1.Department of Animal and Plant SciencesUniversity of Sheffield, Western BankSheffieldUK
  2. 2.Department of Biological SciencesSimon Fraser UniversityBurnabyCanada