Isolation, characterization and predicted genome locations of ruff (Philomachus pugnax, AVES) microsatellite loci
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- Farrell, L.L., Dawson, D.A., Horsburgh, G.J. et al. Conservation Genet Resour (2012) 4: 763. doi:10.1007/s12686-012-9639-0
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We identified 247 unique ruff (Philomachus pugnax) microsatellite sequences. Primer sets were designed from 102 selected loci and tested in 12–24 individuals from a captive population. Sequence homology was used to assign locations in the chicken (Gallus gallus) and/or zebra finch (Taeniopygia guttata) genome for the majority of these loci. Fifty-two loci were found to be polymorphic and 47 of these were typed in known families. Forty-six loci displayed Mendelian inheritance including Ppu058, which was confirmed to be Z-linked by the complete absence of any heterozygous females.