, Volume 4, Issue 3, pp 763-771
Date: 26 May 2012

Isolation, characterization and predicted genome locations of ruff (Philomachus pugnax, AVES) microsatellite loci

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Abstract

We identified 247 unique ruff (Philomachus pugnax) microsatellite sequences. Primer sets were designed from 102 selected loci and tested in 12–24 individuals from a captive population. Sequence homology was used to assign locations in the chicken (Gallus gallus) and/or zebra finch (Taeniopygia guttata) genome for the majority of these loci. Fifty-two loci were found to be polymorphic and 47 of these were typed in known families. Forty-six loci displayed Mendelian inheritance including Ppu058, which was confirmed to be Z-linked by the complete absence of any heterozygous females.