Conservation Genetics Resources

, Volume 4, Issue 3, pp 621–624

Development and characterization of 13 new, and cross amplification of 3, polymorphic nuclear microsatellite loci in the common myna (Acridotheres tristis)

Authors

    • DST-NRF Centre of Excellence for Invasion Biology, Department of Botany and ZoologyStellenbosch University
  • Phillip Cassey
    • Australian Centre for Evolutionary Biology and Biodiversity, School of Earth and Environmental ScienceUniversity of Adelaide
  • Bettine Jansen van Vuuren
    • DST-NRF Centre of Excellence for Invasion Biology, Department of ZoologyUniversity of Johannesburg
  • Berndt Janse van Rensburg
    • DST-NRF Centre of Excellence for Invasion Biology, Department of Zoology and EntomologyUniversity of Pretoria
  • Cang Hui
    • DST-NRF Centre of Excellence for Invasion Biology, Department of Botany and ZoologyStellenbosch University
  • Michael G. Gardner
    • Australian Centre for Evolutionary Biology and Biodiversity, School of Earth and Environmental ScienceUniversity of Adelaide
    • School of Biological SciencesFlinders University
  • Johannes J. Le Roux
    • DST-NRF Centre of Excellence for Invasion Biology, Department of Botany and ZoologyStellenbosch University
Technical Note

DOI: 10.1007/s12686-012-9607-8

Cite this article as:
Berthouly-Salazar, C., Cassey, P., van Vuuren, B.J. et al. Conservation Genet Resour (2012) 4: 621. doi:10.1007/s12686-012-9607-8

Abstract

We used a next generation sequencing (NGS) approach to screen for genome-wide nuclear microsatellite loci in the common (Indian) myna, Acridotheres tristis. In addition, markers previously developed for other Sturnidae species were tested for cross-amplification in A. tristis. In total, we identified 20 loci from NGS data and tested 26 loci for cross-amplification. Out of all loci (NGS developed and cross-amplified), 16 unlinked loci showed polymorphism, ranging from 2 to 9 alleles per locus. Test individuals were obtained from the invasive ranges of common myna in South Africa. Overall, expected and observed heterozygosities ranged from 0.089 to 0.802 and from 0.094 to 0.906, respectively. These markers will be used to shed light on invasion genetics and landscape-scale dynamics of invasive A. tristis in South Africa.

Keywords

Acridotheres tristisCommon mynaInvasive speciesMicrosatellite

Copyright information

© Springer Science+Business Media B.V. 2012