Technical Note

Conservation Genetics Resources

, Volume 4, Issue 2, pp 257-260

First online:

The development of 20 microsatellite loci for the Australian marine mollusk, Donax deltoides, through next generation DNA sequencing

  • Adam D. MillerAffiliated withDepartment of Zoology, The University of MelbourneSchool of Life and Environmental Sciences, Deakin University Email author 
  • , Vincent L. VersaceAffiliated withSchool of Information Systems, Deakin University
  • , Ty MatthewsAffiliated withSchool of Life and Environmental Sciences, Deakin University
  • , Kate C. BowieAffiliated withSchool of Life and Environmental Sciences, Deakin University

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Next Generation DNA sequencing was used to develop a suite of microsatellite markers for the marine mollusk, Donax deltoides. A total of 20 polymorphic loci were identified and 12 characterized using 30 individuals from a single population (Venus Bay) in south eastern Australia. We observed moderate to high genetic variation across most loci (mean number of alleles per locus = 7.3; mean heterozygosity = 0.633) with only a single locus (Ddel32) displaying significant deviation from Hardy–Weinberg equilibrium. Marker independence was confirmed with tests for linkage disequilibrium, however two loci were found to be influenced by null alleles. The 10 viable markers characterized in the present study provide a valuable resource for future population genetic assessments and fisheries management of D. deltoides in Australia.


Microsatellite development Next generation DNA sequencing Donax deltoides Marine mollusk