Novel polymorphic nuclear microsatellite markers for Pinus sylvestris L.
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Scots pine (Pinus sylvestris L.) is one of the most widespread forest trees in the world, ranging from southern Mediterranean mountains to eastern Siberia. Ten polymorphic microsatellite loci were isolated from Scots pine cDNA sequences and were screened for variability in three natural populations. High levels of genetic variability were observed with effective number of alleles per locus ranging from 1.0 to 4.6 and average expected heterozygosity per population of 0.79. With only two exceptions, Hardy–Weinberg expectations were confirmed. All loci were in linkage equilibrium and there was little evidence for confounding null alleles. These new markers will be used to resolve population structure and gene flow patterns in this major Eurasian forest tree.
- Novel polymorphic nuclear microsatellite markers for Pinus sylvestris L.
Conservation Genetics Resources
Volume 4, Issue 2 , pp 231-234
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- Springer Netherlands
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- Scots pine
- Genetic diversity
- Population genetics
- Author Affiliations
- 1. National Research Council, Plant Genetic Institute, Via Madonna del Piano 10, 50019, Sesto Fiorentino, FI, Italy
- 2. Department of Biology, University of Oulu, 90014, Oulu, Finland
- 3. Biocenter Oulu, University of Oulu, 90014, Oulu, Finland
- 4. Department of Forest Ecology and Genetics, Center of Forest Research, CIFOR-INIA, Carretera de A Coruña Km 7.5, 28040, Madrid, Spain