Conservation Genetics Resources

, Volume 4, Issue 1, pp 117–120

Polymorphic microsatellite loci for the endangered pine hoverfly Blera fallax (Diptera: Syrphidae)

  • E. L. Rotheray
  • M. P. Greminger
  • A. Nater
  • M. Krützen
  • D. Goulson
  • L. F. Bussière
Technical Note

DOI: 10.1007/s12686-011-9488-2

Cite this article as:
Rotheray, E.L., Greminger, M.P., Nater, A. et al. Conservation Genet Resour (2012) 4: 117. doi:10.1007/s12686-011-9488-2

Abstract

We describe eleven polymorphic microsatellite loci developed for Blera fallax using ‘next generation’ 454 whole genome shotgun sequencing, along with conditions for three multiplex PCR reactions. We tested allelic variation on forty B. fallax individuals from Scotland and Sweden. Allelic richness and expected heterozygosity were 3.03 (±0.274) and 0.391 (±0.057) respectively. The number of alleles per locus ranged from 2 to 6. We anticipate that these loci will assist conservation management by allowing the monitoring of translocated populations, estimating effective population size, and assessing population structure and dispersal in Scotland and across Europe.

Keywords

SyrphidPine hoverfly454 pyrosequencingMicrosatelliteSSRConservation

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • E. L. Rotheray
    • 1
  • M. P. Greminger
    • 2
  • A. Nater
    • 2
  • M. Krützen
    • 2
  • D. Goulson
    • 1
  • L. F. Bussière
    • 1
  1. 1.Biological and Environmental SciencesUniversity of StirlingStirlingUK
  2. 2.Anthropological Institute and MuseumUniversity of ZürichZürichSwitzerland